Document Detail

Novel resistin promoter polymorphisms: association with serum resistin level in Japanese obese individuals.
MedLine Citation:
PMID:  15326567     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Resistin, an adipocyte-secreted cytokine recently discovered in mice, has been proposed as a link between obesity and diabetes. We analyzed resistin gene polymorphisms and examined their association with serum resistin level and obesity phenotypes in humans. SUBJECTS AND METHODS: Sixty young, obese, non-diabetic subjects taking no medication were studied. DNA sequencing and genotyping of identified single nucleotide polymorphisms were performed. Associations between polymorphisms and serum resistin level, BMI, body composition, fat distribution, and several indices of insulin sensitivity were examined. Moreover, single nucleotide polymorphisms in the promoter region were examined for their influence on resistin gene transcriptional activity using luciferase reporter vectors. RESULTS: Ten non-coding single nucleotide polymorphisms were found. The -638G>A, -420C>G, and -358G>A polymorphisms in the promoter region showed marked linkage disequilibrium with each other, and were associated with serum resistin level; however, there was no association between these polymorphisms and parameters related to adiposity or insulin resistance. The results of luciferase assay revealed that -638G>A together with the -420C>G polymorphism influenced resistin gene transcriptional activity. CONCLUSION: We found that variability in the serum resistin level might be related to polymorphic variants of the promoter region of the gene.
K Azuma; S Oguchi; Y Matsubara; T Mamizuka; M Murata; H Kikuchi; K Watanabe; F Katsukawa; H Yamazaki; A Shimada; T Saruta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  36     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-24     Completed Date:  2005-02-14     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  564-70     Citation Subset:  IM    
Department of Internal Mecicine, School of Medicine, Keio University, Tokyo, Japan.
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MeSH Terms
Asian Continental Ancestry Group / genetics*
Hormones, Ectopic / blood*,  genetics*
Linkage Disequilibrium
Obesity / blood*,  genetics*
Polymorphism, Genetic*
Polymorphism, Single Nucleotide
Promoter Regions, Genetic / genetics*
Transcription, Genetic
Reg. No./Substance:
0/Hormones, Ectopic; 0/RETN protein, human; 0/Resistin; 71-30-7/Cytosine; 73-24-5/Adenine; 73-40-5/Guanine

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