Document Detail

Novel regulatory loci controlling oxygen- and pH-regulated gene expression in Salmonella typhimurium.
MedLine Citation:
PMID:  3276666     Owner:  NLM     Status:  MEDLINE    
Three new loci were discovered, each of which participates in the regulation of anaerobic gene expression. The regulatory gene earA negatively regulates the expression of the anaerobiosis-inducible gene aniG as well as that of at least three other genes, as determined by two-dimensional polyacrylamide gel electrophoresis. The earA locus maps at 86 min. The expression of aniG was also shown to be controlled by changes in external pH under aerobic and anaerobic conditions. Maximal expression was observed under anaerobic conditions at an external pH of 6.0. Significant transcriptional activity was also observed under aerobic conditions at pH 6.0. This was in contrast to hyd, whose expression was dependent upon anaerobiosis and varied with external pH. The pH dependence disappeared under fully aerobic conditions. Mutations in earA had no effect upon hyd expression. The two other regulators identified were oxrF, which controls aniH, and oxrG, which, in concert with oxrA and oxrB, controls aniC and aniI. The oxrG locus was mapped to 88 min and appears to code for a positive regulator. Various oxr mutants were subjected to two-dimensional polyacrylamide electrophoretic analysis of anaerobiosis-inducible proteins. Several pathways of anaerobic control were observed by means of these techniques.
Z Aliabadi; Y K Park; J L Slonczewski; J W Foster
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-10     Completed Date:  1988-03-10     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  842-51     Citation Subset:  IM    
Department of Microbiology, College of Medicine, University of South Alabama, Mobile 36688.
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MeSH Terms
Bacterial Proteins / analysis
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation*
Genes, Bacterial
Genes, Regulator*
Hydrogen-Ion Concentration
Oxygen / metabolism*
Salmonella typhimurium / genetics*,  metabolism
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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