| Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha. | |
MedLine Citation:
|
PMID: 12725926 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A novel class of biopolymers referred to as polythioesters (PTE) was recently detected when the polyhydroxyalkanoate (PHA) accumulating bacterium Ralstonia eutropha was cultivated in the presence of 3-mercaptopropionic acid (3MP) or 3,3'-thiodipropionic acid (TDP). In this study, 3,3'-dithiodipropionic acid (DTDP) and 3-mercaptovaleric acid (3MV) were identified as two additional precursor carbon sources for in vivo biosynthesis of PTE in R. eutropha. Biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3MP, which contributed 19-25% of cell dry matter, was compared referring to the different precursor substrates. Using DTDP as carbon source, which is probably cleaved into two molecules 3MP, yielded an about 2.3-fold higher molar 3MP content of the copolyester than TDP, which is probably cleaved into only one molecule 3MP. Furthermore, cultivation of R. eutropha in the presence of 3MV resulted in biosynthesis of copolymers consisting predominantly of 3HB with low amounts of 3MV and 3-hydroxyvalerate, each contributing less than 5 mol% of the constituents. In contrast, 4-mercaptobutyric acid could be not incorporated into PHAs, although - as documented in this study - five different strategies, various precursor substrates, R. eutropha and also a recombinant strain of Escherichia coli were employed. Therefore, this study not only extended the range of substrates suitable for PTE biosynthesis and also the range of PTE constituents in R. eutropha, it also demonstrates limits for PTE biosynthesis in this bacterium. |
Authors:
|
Tina Lütke-Eversloh; Alexander Steinbüchel |
Related Documents
:
|
24272726 - Do growth substances regulate the phloem as well as the xylem transport of nutrients to... 24704176 - Role of karanja deoiled cake based medium in production of protease and fatty acids by ... 24050256 - Nematicidal activity of mint aqueous extracts against the root-knot nematode meloidogyn... 21700966 - {omega}-3 fatty acids have no impact on serum lactate levels after major gastric cancer... 25100546 - Efficient and selective formation of unsaturated carboxylic and phenylacetic acids from... 22944176 - Ragworm fatty acid profiles reveals habitat and trophic interactions with halophytes an... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 221 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2003 Apr |
Date Detail:
|
Created Date: 2003-05-02 Completed Date: 2003-10-27 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 191-6 Citation Subset: IM |
Affiliation:
|
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3-Mercaptopropionic Acid
/
metabolism Butyrates / metabolism Cupriavidus necator / growth & development, metabolism* Polyesters / chemistry, metabolism* Polymers / chemistry, metabolism* Propionic Acids / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Butyrates; 0/Polyesters; 0/Polymers; 0/Propionic Acids; 107-96-0/3-Mercaptopropionic Acid; 111-17-1/thiodipropionic acid; 13095-66-4/4-mercaptobutyrate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inhibitory effect of L-pyroglutamate on extremophiles: correlation with growth temperature and pH.
Next Document: Expression of the yeast calcineurin subunits CNA1 and CNA2 during growth and hyper-osmotic stress.