Document Detail


Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha.
MedLine Citation:
PMID:  12725926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel class of biopolymers referred to as polythioesters (PTE) was recently detected when the polyhydroxyalkanoate (PHA) accumulating bacterium Ralstonia eutropha was cultivated in the presence of 3-mercaptopropionic acid (3MP) or 3,3'-thiodipropionic acid (TDP). In this study, 3,3'-dithiodipropionic acid (DTDP) and 3-mercaptovaleric acid (3MV) were identified as two additional precursor carbon sources for in vivo biosynthesis of PTE in R. eutropha. Biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3MP, which contributed 19-25% of cell dry matter, was compared referring to the different precursor substrates. Using DTDP as carbon source, which is probably cleaved into two molecules 3MP, yielded an about 2.3-fold higher molar 3MP content of the copolyester than TDP, which is probably cleaved into only one molecule 3MP. Furthermore, cultivation of R. eutropha in the presence of 3MV resulted in biosynthesis of copolymers consisting predominantly of 3HB with low amounts of 3MV and 3-hydroxyvalerate, each contributing less than 5 mol% of the constituents. In contrast, 4-mercaptobutyric acid could be not incorporated into PHAs, although - as documented in this study - five different strategies, various precursor substrates, R. eutropha and also a recombinant strain of Escherichia coli were employed. Therefore, this study not only extended the range of substrates suitable for PTE biosynthesis and also the range of PTE constituents in R. eutropha, it also demonstrates limits for PTE biosynthesis in this bacterium.
Authors:
Tina Lütke-Eversloh; Alexander Steinbüchel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  221     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  191-6     Citation Subset:  IM    
Affiliation:
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
3-Mercaptopropionic Acid / metabolism
Butyrates / metabolism
Cupriavidus necator / growth & development,  metabolism*
Polyesters / chemistry,  metabolism*
Polymers / chemistry,  metabolism*
Propionic Acids / metabolism
Chemical
Reg. No./Substance:
0/Butyrates; 0/Polyesters; 0/Polymers; 0/Propionic Acids; 107-96-0/3-Mercaptopropionic Acid; 111-17-1/thiodipropionic acid; 13095-66-4/4-mercaptobutyrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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