| Novel plant and fungal AGP-like proteins in the Medicago truncatula-Glomus intraradices arbuscular mycorrhizal symbiosis. | |
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MedLine Citation:
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PMID: 18709392 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of arbuscular mycorrhizal (AM) fungi to colonise the root apoplast, and in coordination with the plant develop specialised plant-fungal interfaces, is key to successful symbioses. The availability of expressed sequence tags (EST) of the model legume, Medicago truncatula, and AM fungus, Glomus intraradices, permits identification of genes required for development of symbiotic interfaces. The M. truncatula EST database was searched to identify cell surface arabinogalactan-proteins (AGPs) expressed in mycorrhizal roots. Candidate genes were characterised and gene expression tested using reverse transcription polymerase chain reaction and promoter:reporter gene fusions. Genes encoding one plant AGP and three AGP-like (AGL) proteins (from G. intraradices) were identified. AGL proteins encoded by two AGL genes from G. intraradices (GiAGLs) represent a new structural class of AGPs not found in non-AM fungi or plants. Two GiAGLs differ from plant AGPs by containing charged repeats. Structural modelling shows that GiAGL1 can form a polyproline II helix with separate positively and negatively charged faces, whereas GiAGL3 is charged on all three faces. The unique structural properties of the newly discovered AGLs suggests that they could assist the formation of symbiotic interfaces through self-assembly and interactions with plant cell surfaces. |
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Authors:
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Carolyn J Schultz; Maria J Harrison |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-08-16 |
Journal Detail:
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Title: Mycorrhiza Volume: 18 ISSN: 0940-6360 ISO Abbreviation: Mycorrhiza Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-08 Completed Date: 2009-01-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100955036 Medline TA: Mycorrhiza Country: Germany |
Other Details:
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Languages: eng Pagination: 403-12 Citation Subset: IM |
Affiliation:
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School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia. carolyn.schultz@adelaide.edu.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Fungal Proteins* / chemistry, genetics Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Glomeromycota* / metabolism, physiology Glycosylphosphatidylinositols Medicago truncatula* / metabolism, microbiology Models, Molecular Mucoproteins* / chemistry, genetics Mycorrhizae / physiology* Peptides / chemistry Plant Proteins* / chemistry, genetics Plant Roots / metabolism Protein Structure, Tertiary Recombinant Fusion Proteins / metabolism Symbiosis / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Glycosylphosphatidylinositols; 0/Mucoproteins; 0/Peptides; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 0/arabinogalactan proteins; 25191-13-3/polyproline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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