Document Detail


Novel placental expression of 2,3-bisphosphoglycerate mutase.
MedLine Citation:
PMID:  16246416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
2,3-Bisphosphoglycerate mutase (2,3-BPGM), an erythroid-expressed enzyme, synthesises 2,3-bisphosphoglycerate (2,3-BPG), the allosteric modulator of haemoglobin. This ligand has a higher affinity for adult haemoglobin than for fetal haemoglobin and differential binding of it facilitates transfer of oxygen between adult and fetal blood by lowering the affinity of adult haemoglobin for oxygen. This paper reports the discovery that 2,3-BPGM is synthesised in non-erythroid cells of the human placenta. Western blot analysis of placental extracts revealed high levels of 2,3-BPGM in the human placenta. Immunohistochemical staining and in situ hybridisation experiments indicated that abundant 2,3-BPGM is present in the syncytiotrophoblast layer of the placental villi at the feto-maternal interface. A cytochemical staining technique showed that the placental 2,3-BPGM is active, indicating that 2,3-BPG is synthesised in the outermost cells of the placenta. These observations demonstrate an unexpected and abundant presence of an enzyme key to oxygen release from adult haemoglobin, at the interface between maternal and fetal circulations.
Authors:
D C Pritlove; M Gu; C A R Boyd; H S Randeva; M Vatish
Related Documents :
19396506 - Obstetric hemorrhage during an exit procedure for severe fetal airway obstruction.
12124686 - Shortened fetal long bones: a possible in utero manifestation of placental function.
2701106 - Factors affecting gas transfer across the placenta and the oxygen supply to the fetus.
3335216 - Metabolism of cortisol and cortisone in the baboon fetus at midgestation.
19412806 - Maternal mortality and severe morbidity from sepsis in the netherlands.
11314836 - Glyceryl trinitrate and acute uterine relaxation: a literature review.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-24
Journal Detail:
Title:  Placenta     Volume:  27     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2006-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  924-7     Citation Subset:  IM    
Affiliation:
Clinical Sciences Research Institute, University Hospitals Coventry and Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bisphosphoglycerate Mutase / analysis*,  genetics,  metabolism
Blotting, Western
Female
Humans
Immunohistochemistry
In Situ Hybridization
Placenta / enzymology*
RNA, Messenger / analysis
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 5.4.2.4/Bisphosphoglycerate Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inflammatory mediator release in normal bronchial smooth muscle cells is altered by pregnant materna...
Next Document:  Arginine vasopressin does not contribute to seizures induced by intracerebroventricularly-injected p...