Document Detail

Novel placental expression of 2,3-bisphosphoglycerate mutase.
MedLine Citation:
PMID:  16246416     Owner:  NLM     Status:  MEDLINE    
2,3-Bisphosphoglycerate mutase (2,3-BPGM), an erythroid-expressed enzyme, synthesises 2,3-bisphosphoglycerate (2,3-BPG), the allosteric modulator of haemoglobin. This ligand has a higher affinity for adult haemoglobin than for fetal haemoglobin and differential binding of it facilitates transfer of oxygen between adult and fetal blood by lowering the affinity of adult haemoglobin for oxygen. This paper reports the discovery that 2,3-BPGM is synthesised in non-erythroid cells of the human placenta. Western blot analysis of placental extracts revealed high levels of 2,3-BPGM in the human placenta. Immunohistochemical staining and in situ hybridisation experiments indicated that abundant 2,3-BPGM is present in the syncytiotrophoblast layer of the placental villi at the feto-maternal interface. A cytochemical staining technique showed that the placental 2,3-BPGM is active, indicating that 2,3-BPG is synthesised in the outermost cells of the placenta. These observations demonstrate an unexpected and abundant presence of an enzyme key to oxygen release from adult haemoglobin, at the interface between maternal and fetal circulations.
D C Pritlove; M Gu; C A R Boyd; H S Randeva; M Vatish
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-24
Journal Detail:
Title:  Placenta     Volume:  27     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2006-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  924-7     Citation Subset:  IM    
Clinical Sciences Research Institute, University Hospitals Coventry and Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, United Kingdom.
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MeSH Terms
Bisphosphoglycerate Mutase / analysis*,  genetics,  metabolism
Blotting, Western
In Situ Hybridization
Placenta / enzymology*
RNA, Messenger / analysis
Reg. No./Substance:
0/RNA, Messenger; EC Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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