Document Detail


A novel phage element of Salmonella enterica serovar Enteritidis P125109 contributes to accelerated type III secretion system 2-dependent early inflammation kinetics in a mouse colitis model.
MedLine Citation:
PMID:  22753379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella enterica subsp. I serovar Enteritidis exhibits type III secretion system 2 (TTSS2)-dependent early colonization and inflammation kinetics faster than those of closely related S. enterica serovar Typhimurium. To investigate the accelerated TTSS-2-dependent pathogenic potential of S. Enteritidis, we focused on its genome. Results of a previously published comparative genomic study revealed the presence of mutually exclusive genes in both serovars. In this study, we investigated the roles of six S. Enteritidis-specific genes in vivo by using differential fluorescence induction (DFI) through putative gene-specific promoters. The promoter construct associated with the gene locus SEN1140 induced green fluorescent protein (GFP) expression in the gut lumen, lamina propria, mesenteric lymph nodes, and related systemic organs. To further investigate the potential role of SEN1140, we compared a SEN1140 deletion mutant with S. Typhimurium in a TTSS1-deficient background. Interestingly, the S. Enteritidis mutant lacking SEN1140 did not show the unique TTSS-2-dependent early colonization and inflammation kinetic phenotype of S. Typhimurium. Consistent with this result, complementation of SEN1140 restored the TTSS-2-dependent accelerated inflammatory potential of S. Enteritidis. This report presents a suitable screening strategy that uses a combination of DFI, fluorescence-activated cell sorting, quantitative PCR, and wild-type isogenic tagged-strain techniques to explore the unique roles of S. Enteritidis-specific genes in bacterial pathogenesis.
Authors:
Vikalp Vishwakarma; Balamurugan Periaswamy; Niladri Bhusan Pati; Emma Slack; Wolf-Dietrich Hardt; Mrutyunjay Suar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-02
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-15     Completed Date:  2012-10-24     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3236-46     Citation Subset:  IM    
Affiliation:
School of Biotechnology, Campus-11, KIIT University, Bhubaneswar, Orissa, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Secretion Systems / genetics*
Colitis / microbiology,  pathology*
Disease Models, Animal
Gene Deletion
Genetic Complementation Test
Humans
Mice
Mice, Inbred C57BL
Salmonella Infections, Animal / microbiology,  pathology*
Salmonella Phages / genetics*
Salmonella enteritidis / genetics*,  metabolism,  virology*
Salmonella typhimurium / genetics,  metabolism
Virulence
Virulence Factors / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Virulence Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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