| Novel nonseparation sandwich-type electrochemical enzyme immunoassay system for detecting marker proteins in undiluted blood. | |
| | |
MedLine Citation:
|
PMID: 7544708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A novel nonseparation electrochemical enzyme immunoassay (NEEIA) for detecting marker proteins in undiluted blood is described. The approach is based on preferential electrochemical measurement of surface-bound enzyme-labeled reporter antibody (E-Ab), relative to an excess of this reagent in the sample solution. NEEIAs are carried out on microporous membranes coated with a thin, circular area of gold. The gold serves simultaneously as a working electrode and solid phase for immobilized capture anti-protein antibodies. In the assay, analyte protein is incubated concurrently with the Ab-coated gold surface and excess E-Ab conjugate. Detection of bound E-Ab is achieved by introducing the substrate for the enzyme through the back side of the membrane. The product of bound E-Ab is detected immediately by oxidation or reduction at the gold electrode, and the resulting current is proportional to the concentration of protein in the sample. The feasibility of the NEEIA approach is demonstrated via the detection of prostate-specific antigen in undiluted plasma samples (n = 64), with alkaline phosphatase as the label. Use of multiple gold films deposited on the same porous membrane to perform simultaneous NEEIAs is also described. |
| | |
Authors:
|
M E Meyerhoff; C Duan; M Meusel |
Related Documents
:
|
15538788 - Photoimmobilization of proteins for affinity capture combined with maldi tof ms analysis. 9491348 - Comparison of protein surfaces using a genetic algorithm. 11963898 - Selection of stabilizing additive for lipase immobilization on controlled pore silica b... 23354818 - In silico analysis reveals the presence of a large number of ankyrin repeat containing ... 17942568 - Identification of braf as a new interactor of plcepsilon1, the protein mutated in nephr... 3628238 - Release of individual enzymes from guinea pig pancreas following stimulation with cck-8... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Clinical chemistry Volume: 41 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1995 Sep |
Date Detail:
|
Created Date: 1995-10-03 Completed Date: 1995-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1378-84 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, University of Michigan, Ann Arbor 48109, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal Biological Markers / blood* Blood Proteins / analysis* Chorionic Gonadotropin / blood Electrochemistry Electrodes Gold Humans Immunoenzyme Techniques* Immunoglobulin G Oxidation-Reduction Prostate-Specific Antigen / blood |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Blood Proteins; 0/Chorionic Gonadotropin; 0/Immunoglobulin G; 7440-57-5/Gold; EC 3.4.21.77/Prostate-Specific Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: HPLC-microparticle enzyme immunoassay specific for tacrolimus in whole blood of hepatic and renal tr...
Next Document: Distribution of neuropeptide-containing nerve fibers in the synovium and adjacent bone of the rat kn...