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Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.
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MedLine Citation:
PMID:  3299258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DNA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for beta-Nerve Growth Factor to 2p24 and 1p13 respectively.
Authors:
J A Garson; J A van den Berghe; J T Kemshead
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  15     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-10     Completed Date:  1987-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4761-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase
Azure Stains
Bacterial Proteins
Base Sequence
Biotin
Chromosome Banding / methods
Chromosome Mapping / methods*
DNA, Recombinant
Genes
Humans
Nerve Growth Factors / genetics*
Nucleic Acid Hybridization*
Oncogenes
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-myc
Streptavidin
Chemical
Reg. No./Substance:
0/Azure Stains; 0/Bacterial Proteins; 0/DNA, Recombinant; 0/Nerve Growth Factors; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myc; 58-85-5/Biotin; 9013-20-1/Streptavidin; EC 3.1.3.1/Alkaline Phosphatase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 6 Year: 1987
Volume: 15 Issue: 12
First Page: 4761 Last Page: 4770
ID: 305916
PubMed Id: 3299258

Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.
J A Garson
J A van den Berghe
J T Kemshead



Article Categories:
  • Research Article


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