Document Detail

Novel nanostructural photosensitizers for photodynamic therapy: In vitro studies.
MedLine Citation:
PMID:  22525077     Owner:  NLM     Status:  Publisher    
Photosensitizing properties of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP) functionalized by covalent attachment of one chain of poly(ethylene glycol) (PEG) with a molecular weight of 350, 2000, or 5000Da (p-THPP-PEG(350), p-THPP-PEG(2000), p-THPP-PEG(5000)) were studied in vitro. Dark and photo cytotoxicity of these photosensitizers delivered in solution or embedded in liposomes were evaluated on two cell lines: a human colorectal carcinoma cell line (HCT 116) and a prostate cancer cell line (DU 145), and compared with these treated with free p-THPP. The attachment of PEG chains results in the pronounced reduction of the dark cytotoxicity of the parent porphyrin. Cell viability tests have demonstrated that the phototoxicity of pegylated porphyrins is dependent on the length of PEG chain and p-THPP-PEG(2000) exhibited the highest photodynamic efficacy for both cell lines. The encapsulation into liposomes did not improve the PDT effect. However, the liposomal formulation of p-THPP-PEG(2000) showed a greater tendency to induce apoptosis in both cell lines than the parent or pegylated porphyrin delivered in solution. The colocalization of p-THPP, p-THPP-PEG(2000) and p-THPP-PEG(2000) enclosed in liposomes with fluorescent markers for lysosomes, mitochondria, endoplasmatic reticulum (ER) and Golgi apparatus (GA) was determined in the HCT 116 line. The p-THPP exhibited ubiquitous intracellular distribution with a preference for membranes: mitochondria, ER, GA, lysosomes and plasma membrane. Fluorescence of p-THPP-PEG(2000) was observed within the cytoplasm, with a stronger signal detected in membranous organelle: mitochondria, ER, GA and lysosomes. In contrast, p-THPP-PEG(2000) delivered in liposomes gave a distinct lysosomal pattern of localization.
Kinga Nawalany; Aleksandra Rusin; Mariusz Kepczynski; Piotr Filipczak; Marta Kumorek; Bartłomiej Kozik; Hana Weitman; Benjamin Ehrenberg; Zdzisław Krawczyk; Maria Nowakowska
Related Documents :
1385517 - Monoclonal antibodies specific to quail embryo tissues: their epitopes in the developin...
16656847 - Studies on cytokinin-controlled bud formation in moss protonemata.
3161767 - The relationship of nicotinamide adenine dinucleotide to the chondrogenic differentiati...
1729137 - Evidence for resegmentation in the formation of the vertebral column using the novel ap...
21045567 - Formation of microvilli and phosphorylation of erm family proteins by cd43, a potent in...
19297027 - Manganese(iii)-salens induce tumor selective apoptosis in human cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-15
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  -     ISSN:  1873-3476     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland; Faculty of Commodity Science, Cracow University of Economics, Rakowicka 27, 31-510 Kraków, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enhanced antitumor efficacy, biodistribution and penetration of docetaxel-loaded biodegradable nanop...
Next Document:  Agitation during lipoplex formation harmonizes the interaction of siRNA to cationic liposomes.