| Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cells. | |
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MedLine Citation:
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PMID: 12475956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have cloned a full-length cDNA encoding a novel myosin II heavy chain kinase (mhckC) from Dictyostelium. Like other members of the myosin heavy chain kinase family, the mhckC gene product, MHCK C, has a kinase domain in its N-terminal half and six WD repeats in the C-terminal half. GFP-MHCK C fusion protein localized to the cortex of interphase cells, to the cleavage furrow of mitotic cells, and to the posterior of migrating cells. These distributions of GFP-MHCK C always corresponded with that of myosin II filaments and were not observed in myosin II-null cells, where GFP-MHCK C was diffusely distributed in the cytoplasm. Thus, localization of MHCK C seems to be myosin II-dependent. Cells lacking the mhckC gene exhibited excessive aggregation of myosin II filaments in the cleavage furrows and in the posteriors of the daughter cells once cleavage was complete. The cleavage process of these cells took longer than that of wild-type cells. Taken together, these findings suggest MHCK C drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during cytokinesis. |
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Authors:
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Akira Nagasaki; Go Itoh; Shigehiko Yumura; Taro Q P Uyeda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular biology of the cell Volume: 13 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-11 Completed Date: 2003-07-29 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 4333-42 Citation Subset: IM |
Affiliation:
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Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Ibaraki 305-8562, Japan. a-nagasaki@aist.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases / biosynthesis*, physiology* Catalytic Domain Cell Division DNA, Complementary / metabolism Dictyostelium / metabolism* Green Fluorescent Proteins Luminescent Proteins / metabolism Microscopy, Fluorescence Mitosis Models, Biological Models, Genetic Molecular Sequence Data Myosin Type II / chemistry*, metabolism Protein Structure, Tertiary Protozoan Proteins Reverse Transcriptase Polymerase Chain Reaction Time Factors |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Luminescent Proteins; 0/Protozoan Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.7/myosin-heavy-chain kinase; EC 3.6.1.-/Myosin Type II |
| Comments/Corrections | |
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