Document Detail


Novel microtiter plate format for testing germ tube formation and proposal of a cost-effective scheme for yeast identification in a clinical laboratory.
MedLine Citation:
PMID:  10626129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The germ tube test is most widely used for presumptive identification of Candida albicans. Conventional testing is relatively time-consuming due to the hands-on time involved in preparing and viewing each isolate. In order to reduce work-load and costs we have developed a novel microtiter plate test format that offers several advantages: (i) use of removable strips of microtiter wells placed in lockwell frames, (ii) only one pipetting step for each isolate, (iii) direct micromorphological evaluation using an inverse microscope, (iv) use of a novel synthetic germ tube test medium, (v) reduction of the inoculum, permitting testing of minute colonies, (vi) thus, testing of different colonies in potentially mixed primary cultures of clinical specimens is encouraged and facilitated. Implementing this microtiter based germ tube test with simultaneous trehalase test for presumptive identification of Torulopsis (Candida) glabrata, we propose an identification scheme including this test format. This has been implemented in our routine laboratory permitting cost-effective presumptive identification of almost all clinically relevant yeast species.
Authors:
H Peltroche-Llacsahuanga; A Jenster; R Lütticken; G Haase
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  35     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-02-23     Completed Date:  2000-02-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  197-204     Citation Subset:  IM    
Affiliation:
Institute of Medical Microbiology, University Hospital, RWTH Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Candida / classification*,  growth & development,  isolation & purification
Candidiasis / microbiology*
Cost-Benefit Analysis
Culture Media
Humans
Laboratories, Hospital
Mycological Typing Techniques / economics*
Mycology / methods*
Trehalase / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; EC 3.2.1.28/Trehalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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