| Novel microtiter plate format for testing germ tube formation and proposal of a cost-effective scheme for yeast identification in a clinical laboratory. | |
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MedLine Citation:
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PMID: 10626129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The germ tube test is most widely used for presumptive identification of Candida albicans. Conventional testing is relatively time-consuming due to the hands-on time involved in preparing and viewing each isolate. In order to reduce work-load and costs we have developed a novel microtiter plate test format that offers several advantages: (i) use of removable strips of microtiter wells placed in lockwell frames, (ii) only one pipetting step for each isolate, (iii) direct micromorphological evaluation using an inverse microscope, (iv) use of a novel synthetic germ tube test medium, (v) reduction of the inoculum, permitting testing of minute colonies, (vi) thus, testing of different colonies in potentially mixed primary cultures of clinical specimens is encouraged and facilitated. Implementing this microtiter based germ tube test with simultaneous trehalase test for presumptive identification of Torulopsis (Candida) glabrata, we propose an identification scheme including this test format. This has been implemented in our routine laboratory permitting cost-effective presumptive identification of almost all clinically relevant yeast species. |
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Authors:
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H Peltroche-Llacsahuanga; A Jenster; R Lütticken; G Haase |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Diagnostic microbiology and infectious disease Volume: 35 ISSN: 0732-8893 ISO Abbreviation: Diagn. Microbiol. Infect. Dis. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-02-23 Completed Date: 2000-02-23 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8305899 Medline TA: Diagn Microbiol Infect Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 197-204 Citation Subset: IM |
Affiliation:
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Institute of Medical Microbiology, University Hospital, RWTH Aachen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Candida
/
classification*,
growth & development,
isolation & purification Candidiasis / microbiology* Cost-Benefit Analysis Culture Media Humans Laboratories, Hospital Mycological Typing Techniques / economics* Mycology / methods* Trehalase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; EC 3.2.1.28/Trehalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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