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Novel method for studying postoperative ileus in mice.
MedLine Citation:
PMID:  23320135     Owner:  NLM     Status:  PubMed-not-MEDLINE    
INTRODUCTION: Postoperative ileus (POI) is characterized by a transient inhibition of coordinated motility of the gastrointestinal (GI) tract after abdominal surgery and leads to increased morbidity and prolonged hospitalization. Currently, intestinal manipulation of the intestine is widely used as a preclinical model of POI. The technique used to manipulate the intestine is however highly variable and difficult to standardize, leading to large variations and inconsistent findings between different investigators. Therefore, we developed a device by which a fixed and adjustable pressure can be applied during intestinal manipulation.
METHODS: The standardized pressure manipulation method was developed using the purpose-designed device. First, the effect of graded manipulation was examined on postoperative GI transit. Next, this new technique was compared to the conventional manipulation technique used in previous studies. GI transit was measured by evaluating the intestinal distribution of orally gavaged fluorescein isothiocyanate (FITC)-labeled dextran. Infiltration of myeloperoxidase positive cells and cytokine production (ELISA) in the muscularis externa of the intestine were assessed.
RESULTS: Increasing pressures resulted in a graded reduction of intestinal transit and was associated with intestinal inflammation as demonstrated by influx of leukocytes and increased levels of IL-6, IL-1β and MCP-1 compared to control mice. With an applied pressure of 9 grams a similar delay in intestinal transit could be obtained with a smaller standard deviation, leading to a reduced intra-individual variation.
CONCLUSIONS: This method provides a reproducible model with small variation to study the pathophysiology of POI and to evaluate new anti-inflammatory strategies.
Sjoerd Hw van Bree; Andrea Nemethova; Fleur S van Bovenkamp; Pedro Gomez-Pinilla; L Elbers; Martina Di Giovangiulio; Gianluca Matteoli; Jan van Vliet; Cathy Cailotto; Michael Wt Tanck; Guy Ee Boeckxstaens
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Publication Detail:
Type:  Journal Article     Date:  2012-12-26
Journal Detail:
Title:  International journal of physiology, pathophysiology and pharmacology     Volume:  4     ISSN:  1944-8171     ISO Abbreviation:  Int J Physiol Pathophysiol Pharmacol     Publication Date:  2012  
Date Detail:
Created Date:  2013-01-15     Completed Date:  2013-01-16     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101521074     Medline TA:  Int J Physiol Pathophysiol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-27     Citation Subset:  -    
Tytgat institute of Liver and Intestinal Research, Department of Gastroenterology & Hepatology, Academic Medical Center Amsterdam, the Netherlands.
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