Document Detail


Novel mdm2 splice variants identified in pediatric rhabdomyosarcoma tumors and cell lines.
MedLine Citation:
PMID:  11939408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mdm2 is an oncogene that binds to and inactivates the tumor suppressor p53. However, the presence of oncogenic splice variants of mdm2 in human tumors that lack the p53 binding site has suggested a p53-independent transforming function for this protein. This report describes expression of 11 different mdm2 splice variants in pediatric rhabdomyosarcoma (RMS) cell lines and tumors at a frequency of 75% and 82%, respectively. Five of these isoforms have previously been described in other tumor histiotypes but six are novel and may be unique to RMS. There was no association between expression of splice variants and mdm2 gene amplification or p53 status. In addition, the frequency of splice variants was much higher than the incidence of mdm2 amplification or p53 mutations. These variants may be important to consider with respect to RMS tumor progression and therapeutic response.
Authors:
F Bartel; A C Taylor; H Taubert; L C Harris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncology research     Volume:  12     ISSN:  0965-0407     ISO Abbreviation:  Oncol. Res.     Publication Date:  2001  
Date Detail:
Created Date:  2002-04-09     Completed Date:  2002-09-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9208097     Medline TA:  Oncol Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  451-7     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing*
Blotting, Northern
Child
Genetic Variation*
Humans
Neoplasm Proteins / genetics
Neoplasm Staging
Nuclear Proteins*
Oncogenes
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-mdm2
RNA, Neoplasm / genetics
Reverse Transcriptase Polymerase Chain Reaction
Rhabdomyosarcoma / genetics*,  pathology
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA23099/CA/NCI NIH HHS; CA77541/CA/NCI NIH HHS; P30-CA21765/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Nuclear Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Neoplasm; EC 6.3.2.19/MDM2 protein, human; EC 6.3.2.19/Proto-Oncogene Proteins c-mdm2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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