Document Detail


Novel markers of cell kinetics to evaluate progression from cirrhosis to hepatocellular carcinoma.
MedLine Citation:
PMID:  16629645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We investigated cell cycle kinetics of nodular lesions in cirrhosis to differentiate hepatocellular carcinoma (HCC) from its precursor lesions. METHODS: Twelve small HCC, 10 regenerative (RN), six large regenerative (LRN), and five dysplastic nodules (DN), identified in explant cirrhotic livers of five consecutive patients transplanted at Royal Free Hospital in 2002. Immunoperoxidase for MCM2, geminin and Ki67 was performed and the percentage of positive cells counted. RESULTS: The proportion of cells expressing MCM2 was more than those expressing Ki67, which in turn was more than those expressing geminin (overall median=16%, 2% and 0.5%, respectively, P<0.001). There was a statistically significant trend of increasing Ki67 expression (P=0.006), from RN to HCC; this trend was not statistically significant for geminin (P=0.18) or MCM2 (P=0.51). The median percentage of cells expressing Ki67 was 1% in RN, 0.5% in LRN, 2.2% in DN and 5.4% in HCC. The combination of these markers identified four different cell kinetics patterns: 'resting' (G0 cells: MCM2 -ve, Ki67 -ve, geminin -ve); 'licensed' (MCM2 +ve, Ki67 -ve, geminin -ve); 'slowly growing' (G1 phase arrest, MCM2 +ve, Ki67 +ve, low (0.4%) geminin) and expanding (MCM2 +ve, Ki67 +ve, geminin +ve) nodules. CONCLUSIONS: The combination of MCM2, geminin and Ki67 could represent a valuable tool in the understanding of HCC progression in cirrhosis.
Authors:
Alberto Quaglia; Mary McStay; Kai Stoeber; Marco Loddo; Martyn Caplin; Thomas Fanshawe; Gareth Williams; Amar Dhillon
Related Documents :
15040015 - Downregulation of retinoic acid receptor-beta(2) expression is linked to aberrant methy...
3607755 - Sex hormone response of a newly established squamous cell line derived from clinical es...
222435 - Influence of cell type on failure pattern after irradiation for locally advanced carcin...
6165245 - Pale cell carcinoma: ultrastructure of a hormone-dependent mammary tumor in gr mice.
7150405 - Adenovirus-cell interactions early after infection: in vitro characteristics and tumour...
16005685 - Transduction of cpg dna-stimulated primary human b cells with bicistronic lentivectors.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Liver international : official journal of the International Association for the Study of the Liver     Volume:  26     ISSN:  1478-3223     ISO Abbreviation:  Liver Int.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-24     Completed Date:  2006-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160857     Medline TA:  Liver Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  424-32     Citation Subset:  IM    
Affiliation:
Department of Histopathology, Royal Free and University College Medical School, London, UK. Alberto.Quaglia@kingsch.nhs.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers
Carcinoma, Hepatocellular / genetics,  metabolism,  physiopathology*
Cell Cycle*
Cell Cycle Proteins / genetics,  metabolism
Cell Transformation, Neoplastic
Disease Progression
Gene Expression Regulation
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Ki-67 Antigen / genetics,  metabolism
Kinetics
Liver Cirrhosis / genetics,  metabolism,  physiopathology*
Liver Neoplasms / genetics,  metabolism,  physiopathology*
Male
Middle Aged
Nuclear Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cell Cycle Proteins; 0/GMNN protein, human; 0/Ki-67 Antigen; 0/MCM2 protein, human; 0/Nuclear Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression pattern of angiogenic factors and prognosis after hepatic resection in hepatocellular car...
Next Document:  Management of spontaneous haemorrhage and rupture of hepatocellular adenomas. A single centre experi...