| Novel macro-microporous gelatin scaffold fabricated by particulate leaching for soft tissue reconstruction with adipose-derived stem cells. | |
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MedLine Citation:
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PMID: 23143193 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The restoration of body contours as shaped by adipose tissue remains a clinical challenge specifically in patients who have experienced loss of contour due to trauma, surgical removal of tumours or congenital abnormalities. We have developed a novel macro-microporous biomaterial for use in soft tissue re-bulking and augmentation. Alginate beads provided the pore template for the construct. Incorporation, and subsequent dissolution, of the beads within a 7 % (w/v) gelatin matrix, produced a highly porous scaffold with an average pore size of 2.01 ± 0.08 mm. The ability of this scaffold to support the in vitro growth and differentiation of human adipose-derived stem cells (ADSCs) was then investigated. Histological analysis confirmed that the scaffold itself provided a suitable environment to support the growth of ADSCs on the scaffold walls. When delivered into the macropores in a fibrin hydrogel, ADSCs proliferated and filled the pores. In addition, ADSCs could readily be differentiated along the adipogenic lineage. These results therefore describe a novel scaffold that can support the proliferation and delivery of ADSCs. The scaffold is the first stage in developing a clinical alternative to current treatment methods for soft tissue reconstruction. |
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Authors:
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Manraj K Phull; Trevor Eydmann; Judy Roxburgh; Justin R Sharpe; Diana J Lawrence-Watt; Gary Phillips; Yella Martin |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-10 |
Journal Detail:
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Title: Journal of materials science. Materials in medicine Volume: - ISSN: 1573-4838 ISO Abbreviation: J Mater Sci Mater Med Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9013087 Medline TA: J Mater Sci Mater Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Brighton Sussex Medical School, Brighton, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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