| Novel kruppel-like factor is induced by neuronal activity and by sensory input in the central nervous system of the terrestrial slug Limax valentianus. | |
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MedLine Citation:
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PMID: 16288475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the Limax central nervous system, the procerebrum is thought to be the locus of odor information processing and odor memory retention, but little is known about the input pathway of the noxious stimuli used in this learning protocol. To gain insight into the sensory information processing of the noxious stimuli involved in memory formation, we screened genes induced by artificial neuronal activity, and identified one kruppel-like factor (KLF) family transcription factor gene (Limax KLF; limKLF), which is up-regulated 30 min after depolarization. The limKLF protein fused to GFP was localized to the nucleus in COS-7 cells. We also cloned an immediate early gene, CCAAT enhancer binding protein (C/EBP), of Limax by reverse transcription-polymerase chain reaction (RT-PCR). Both genes were up-regulated by dissection of the central nervous system (CNS) out of the slug in a protein synthesis-independent manner, and also by various noxious stimuli to the slug's body. These genes may be useful as neuronal activity markers in Limax to visualize activated sensory nervous systems. |
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Authors:
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Satoshi Fukunaga; Ryota Matsuo; Syohei Hoshino; Yutaka Kirino |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurobiology Volume: 66 ISSN: 0022-3034 ISO Abbreviation: J. Neurobiol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2005-12-28 Completed Date: 2006-04-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0213640 Medline TA: J Neurobiol Country: United States |
Other Details:
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Languages: eng Pagination: 169-81 Citation Subset: IM |
Affiliation:
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Laboratory of Neurobiophysics, School of Pharmaceutical Sciences, The University of Tokyo, 7 Bunkyo-ku, Tokyo 113-0033, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB185103; AB185104 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Aplysia / genetics Base Sequence Blotting, Northern Blotting, Western CCAAT-Enhancer-Binding Proteins / metabolism COS Cells Central Nervous System / physiology* Cercopithecus aethiops Gene Expression Gene Expression Profiling Immunohistochemistry In Situ Hybridization Kruppel-Like Transcription Factors / metabolism* Learning / physiology* Molecular Sequence Data Mollusca / genetics* Neurons, Afferent / physiology* Reverse Transcriptase Polymerase Chain Reaction Sequence Homology |
| Chemical | |
Reg. No./Substance:
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0/CCAAT-Enhancer-Binding Proteins; 0/Kruppel-Like Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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