| Novel insights into the use of Glowing LNA as nucleic acid detection probes--influence of labeling density and nucleobases. | |
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MedLine Citation:
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PMID: 21071224 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Appropriately designed 2'-N-(pyren-1-yl)carbonyl-2'-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes. |
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Authors:
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Michael E Østergaard; Jyotirmoy Maity; B Ravindra Babu; Jesper Wengel; Patrick J Hrdlicka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-11-09 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: 20 ISSN: 1464-3405 ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: England |
Other Details:
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Languages: eng Pagination: 7265-8 Citation Subset: IM |
Copyright Information:
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Published by Elsevier Ltd. |
Affiliation:
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Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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