Document Detail

Novel insights into human intestinal epithelial cell proliferation in health and disease using confocal microscopy.
MedLine Citation:
PMID:  7698694     Owner:  NLM     Status:  MEDLINE    
Measurement of intestinal epithelial cell proliferation has provided important information concerning tissue responses in neoplasia, enteropathy, and adaptation. This study reexamined current concepts regarding intestinal proliferation by using a novel confocal microscopical technique to map mitotic figures accurately within the intact three dimensional framework of the crypts of Lieberkühn. The ability of confocal microscopy to simultaneously measure crypt morphology and internal detail, without disrupting spatial cell arrangements, has provided important new data. These question the ability of existing methods to accurately measure and interpret proliferative changes in the gut. This work investigates intestinal proliferation in children with coeliac disease, a well defined hyperproliferative disorder, in comparison with the healthy intestine. These results show that crypt cell division occurs with an equal probability in health and disease. In addition, increased crypt cell production rates are largely caused by a change in crypt size rather than a change in cell cycle time or crypt growth fraction and, as such, alter our understanding of kinetic responses in gastrointestinal disease.
T C Savidge; J A Walker-Smith; A D Phillips
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gut     Volume:  36     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-04     Completed Date:  1995-05-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  369-74     Citation Subset:  AIM; IM    
Academic Department of Paediatric Gastroenterology, Queen Elizabeth Hospital for Children, Hackney, London.
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MeSH Terms
Celiac Disease / pathology*
Cell Division / physiology
Cell Size
Child, Preschool
Epithelial Cells
Epithelium / pathology
Intestinal Mucosa / cytology*,  pathology
Microscopy, Confocal
Mitotic Index

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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