| Novel histamine measurement by HPLC analysis used to assay histidine decarboxylase inhibitory activity of shoyuflavones from soy sauce. | |
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MedLine Citation:
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PMID: 9757553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An easy and highly sensitive method for measuring histamine by HPLC analysis coupled with precolumn derivatization was established. The amino group of histamine was completely colorimetrically labelled with 4-N,N-dimethylamino-azobenzene-4'-isothiocyanate (DABITC) in the presence of sodium bicarbonate at 90 degrees C for 5 min. The derivative was sensitively and easily analyzed by HPLC on a Cosmosil 5SL column using CHCl3/N,N-dimethylformamide/H2O (210:90:4) containing 0.4% acetic acid. Using the established method, histidine decarboxylase (HDC) inhibitory activities of three tartaric acid isoflavone derivatives, named shoyuflavones, isolated from soy sauce were examined in vitro by measuring the histamine produced by HDC. They showed intense inhibition of the activities of HDC from both mouse mastocytoma P-815 cells and Clostridium perfringens. |
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Authors:
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E Kinoshita; M Saito |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 62 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-10-29 Completed Date: 1998-10-29 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1488-91 Citation Subset: IM |
Affiliation:
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Research and Development Division, Kikkoman Corporation, Chiba, Japan. QZS01157@nifty.ne.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzopyrans / pharmacology Chromatography, High Pressure Liquid Clostridium perfringens / enzymology Enzyme Inhibitors / pharmacology* Fermentation Flavonoids / pharmacology Histamine / analysis* Histidine Decarboxylase / antagonists & inhibitors* Indicators and Reagents / chemistry Isoflavones / pharmacology* Isothiocyanates / chemistry Mice Sensitivity and Specificity Soybeans / metabolism* p-Dimethylaminoazobenzene / analogs & derivatives, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrans; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Indicators and Reagents; 0/Isoflavones; 0/Isothiocyanates; 0/shoyuflavone A; 0/shoyuflavone B; 0/shoyuflavone C; 51-45-6/Histamine; 60-11-7/p-Dimethylaminoazobenzene; 7612-98-8/4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate; EC 4.1.1.22/Histidine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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