| Novel functional biodegradable polymer II: fibroblast growth factor-2 activities of poly(gamma-glutamic acid)-sulfonate. | |
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MedLine Citation:
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PMID: 15638545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Basic fibroblast growth factor (FGF-2) mitogenic activities of sulfonated poly(gamma-glutamic acid) (gamma-PGA-S) were investigated with chlorate-treated L929 fibroblast culture tests. When 72% of the carboxyl groups in gamma-PGA were sulfonated (gamma-PGA-S72), cell numbers reached a maximum. The activity of gamma-PGA-S72 was higher than that of gamma-PGA and synthetic heparinoids and was almost comparable to that of heparin. Cytotoxicity of gamma-PGA-S72 was not observed, regardless of the degree of sulfonation. FGF-2-protective effects of gamma-PGA-S72 against acid and thermal inactivation were also evaluated, and gamma-PGA-S72 showed higher FGF-2-protective effects in comparison to nonsulfonated gamma-PGA. The steric structures of various sulfonated gamma-PGA-Ss were analyzed by molecular modeling (molecular orbital method (MOPAC)) and indicated that gamma-PGA-Ss are helical in vacuo. Results from MOPAC and the molecular mechanics method (MM2) demonstrated that electrostatic interactions can take place between sulfonic and carboxyl groups of gamma-PGA-S and basic amino acid residues in FGF-2. gamma-PGA-S72 can interact with FGF-2 strongly. |
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Authors:
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Michiya Matsusaki; Takeshi Serizawa; Akio Kishida; Mitsuru Akashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 6 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2005 Jan-Feb |
Date Detail:
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Created Date: 2005-01-10 Completed Date: 2005-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 400-7 Citation Subset: IM |
Affiliation:
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Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biodegradation, Environmental Cell Proliferation / drug effects Fibroblast Growth Factor 2 / chemistry, drug effects*, metabolism Fibroblasts / cytology, drug effects* Heparin / pharmacology Hydrogen-Ion Concentration Mice Models, Molecular Polyglutamic Acid / chemical synthesis, chemistry, pharmacology* Polymers / chemistry* Protein Denaturation / drug effects Sulfonic Acids / chemical synthesis, pharmacology* Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 0/Sulfonic Acids; 103107-01-3/Fibroblast Growth Factor 2; 25513-46-6/Polyglutamic Acid; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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