Document Detail


Novel fucogangliosides found in human colon adenocarcinoma tissues by means of glycomic analysis.
MedLine Citation:
PMID:  17350584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structures of acidic glycosphingolipids in colon adenocarcinoma have been analyzed extensively using a number of conventional methods, such as thin-layer chromatography and methylation analysis, and a variety of acidic glycosphingolipids present in the tissues have been reported. However, because of a number of limitations in the techniques used in previous studies in terms of resolution, quantification, and sensitivity, we employed a different method that could be applied to small amounts of tissue. In this technique, the carbohydrate moieties of acidic glycosphingolipids from approximately 20mg of colon adenocarcinoma were released by endoglycoceramidase II and were labeled by pyridylamination. They were separated and structurally characterized by a two-dimensional HPLC mapping technique, electrospray ionization tandem mass spectrometry (ESI-MS/MS), and enzymatic cleavage. A total of 22 major acidic glycosphingolipid structures were identified, and their relative quantities were revealed in detail. They are composed of 1 sulfated (SM3), 1 lacto-series (SLe(a)), 6 kinds of ganglio-series, and 14 kinds of neolacto-series glycosphingolipids. They include most of the acidic glycosphingolipids previously reported to be present in the tissues and two previously unknown fucogangliosides sharing the same terminal structure: NeuAcalpha2-6(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc, and NeuAcalpha2-6(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3-Galbeta1-4Glc. Thus, this highly sensitive, high-resolution analysis enabled the identification of novel structures of acidic glycosphingolipids from small amounts of already comprehensively studied cancerous tissues. This method is a powerful tool for microanalysis of glycosphingolipid structures from small quantities of cancerous tissues and should be applicable to different types of malignant tissues.
Authors:
Hiroaki Korekane; Satoyo Tsuji; Shingo Noura; Masayuki Ohue; Yo Sasaki; Shingi Imaoka; Yasuhide Miyamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-31
Journal Detail:
Title:  Analytical biochemistry     Volume:  364     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-03     Completed Date:  2007-07-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-50     Citation Subset:  IM    
Affiliation:
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-2 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acidic Glycosphingolipids / chemistry,  isolation & purification*
Adenocarcinoma / chemistry*
Aminopyridines / chemistry
Carbohydrate Sequence
Chromatography, High Pressure Liquid / methods
Colonic Neoplasms / chemistry*
Fucose / chemistry
Gangliosides / chemistry,  isolation & purification*
Gels / chemistry
Glycoside Hydrolases / metabolism
Humans
Oligosaccharides, Branched-Chain / chemistry*,  isolation & purification
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Tumor Markers, Biological / chemistry,  isolation & purification*
Chemical
Reg. No./Substance:
0/Acidic Glycosphingolipids; 0/Aminopyridines; 0/Gangliosides; 0/Gels; 0/Oligosaccharides, Branched-Chain; 0/Tumor Markers, Biological; 0/fucogangliosides; 3713-31-3/Fucose; 504-29-0/alpha-aminopyridine; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.123/endoglycoceramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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