Document Detail

Novel fragmentation pathway for CID of (b(n) - 1 + Cat)+ ions from model, metal cationized peptides.
MedLine Citation:
PMID:  15990332     Owner:  NLM     Status:  MEDLINE    
We report a new fragmentation pathway for the CID of (b3 - 1 + Cat)+ product ions derived from the model peptide AXAG, where X = beta-alanine, gamma-aminobutyric acid, epsilon-amino-n-caproic acid, or 4-aminomethylbenzoic acid. By changing the amino acid to the C-terminal side of the amino acid X, and incorporating 15N and 13C labeled residues at the same position, we conclude that the dissociation pathway most likely leads to a metal cationized nitrile. With respect to the various amino acids at position X, the putative nitrile product becomes more prominent, relative to the conventional (a3 - 1 + Cat)+ species, in the order beta-alanine < gamma-aminobutyric acid < epsilon-aminocaproic acid < 4-aminomethylbenzoic acid. The pathway is not observed for peptides with alpha-amino acids at position X. The product ion is observed most prominently during the CID of Li+ and Na+ cationized peptides, only to a small extent for Ag+ cationized peptides, and not at all from protonated analogues.
Travis J Cooper; Erach R Talaty; Michael J Van Stipdonk
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  16     ISSN:  1044-0305     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-01     Completed Date:  2005-10-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1305-10     Citation Subset:  IM    
Department of Chemistry, Wichita State University, Wichita, Kansas 67260-0051, USA.
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MeSH Terms
Cations / analysis,  chemistry
Gas Chromatography-Mass Spectrometry / methods*
Metals / chemistry*
Nitriles / analysis,  chemistry
Peptides / analysis*,  chemistry
Proteomics / instrumentation,  methods*
Reg. No./Substance:
0/Cations; 0/Metals; 0/Nitriles; 0/Peptides; 7439-93-2/Lithium; 7440-23-5/Sodium

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