Document Detail


Novel expression pattern of interphotoreceptor retinoid-binding protein (IRBP) in the adult and developing zebrafish retina and RPE.
MedLine Citation:
PMID:  9841935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Interactions between the neural retina and retinal pigment epithelium (RPE) are mediated by the interphotoreceptor matrix (IPM). The transport of retinoids across the IPM is mediated by interphotoreceptor retinoid-binding protein (IRBP). To explore the possibility that IRBP is important during retinal development, we examined its spatiotemporal expression pattern in embryonic zebrafish. METHODS: IRBP mRNA expression was examined using RT-PCR and in situ hybridization. IRBP was localized using antiserum against recombinant zebrafish IRBP. IRBP synthesis and secretion were studied by in vitro metabolic labeling of retinas and RPE-eyecups. RESULTS: IRBP mRNA was first observed in the pineal at 24 hours post-fertilization (hpf) and in the ventral retina at 50 hpf. Immunoreactive IRBP was first observed at 72 hpf. Remarkably, IRBP was expressed not only by photoreceptors but also by the adult and embryonic RPE. In embryos, expression in both retina and RPE began in a ventronasal patch and spread to involve the entire eye. In general, early IRBP expression was dominated by photoreceptors, but then RPE expression spread beyond the limit of photoreceptor expression. Double in situ hybridizations suggests that cones express IRBP mRNA before they express a specific opsin, while rods may express rod opsin prior to IRBP. CONCLUSIONS: The temporal and spatial patterns of IRBP expression by the RPE and retina are consistent with a role in retinal development and suggest coordination of RPE and photoreceptor differentiation.
Authors:
D L Stenkamp; L L Cunningham; P A Raymond; F Gonzalez-Fernandez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  1998-12-02
Journal Detail:
Title:  Molecular vision     Volume:  4     ISSN:  1090-0535     ISO Abbreviation:  Mol. Vis.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1998-12-30     Completed Date:  1998-12-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9605351     Medline TA:  Mol Vis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  26     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Eye Proteins / metabolism
Fluorescent Antibody Technique, Indirect
In Situ Hybridization
Photoreceptor Cells / metabolism
Pigment Epithelium of Eye / embryology,  metabolism*
RNA, Messenger / metabolism
Retina / embryology,  metabolism*
Retinol-Binding Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Zebrafish / embryology,  metabolism*
Grant Support
ID/Acronym/Agency:
EY 09412/EY/NEI NIH HHS; F32 EY06612/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Eye Proteins; 0/RNA, Messenger; 0/Retinol-Binding Proteins; 0/interstitial retinol-binding protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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