| Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection. | |
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MedLine Citation:
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PMID: 21559870 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a planar substrate. The optical elements are based on supercritical angle fluorescence (SAF) which provides substantial enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume strictly to the immediate proximity of the biochip surface, thereby having the potential to discriminate against background fluorescence from the analyte solution. An optical reader is also described that enables interrogation and fluorescence collection from the nine optical elements on the chip. The sensitivity of the system was determined with a biotin-avidin assay while its clinical utility was demonstrated in an assay for C-reactive protein (CRP), an inflammation marker. |
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Authors:
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Duncan Hill; Barry McDonnell; Stephen Hearty; Lourdes Basabe-Desmonts; Robert Blue; Michal Trnavsky; Colm McAtamney; Richard O'Kennedy; Brian D Maccraith |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-11 |
Journal Detail:
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Title: Biomedical microdevices Volume: - ISSN: 1572-8781 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100887374 Medline TA: Biomed Microdevices Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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