| Novel diffusion cell for in vitro transdermal permeation, compatible with automated dynamic sampling. | |
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MedLine Citation:
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PMID: 8818009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of a new diffusion cell for in vitro transdermal permeation is described. The so-called Kelder cells were used in combination with the ASPEC system (Automatic Sample Preparation with Extraction Columns), which is designed for the automation of solid-extractions (SPE). Instead of SPE columns, 20 Kelder cells were placed in the racks. This allowed automatic sampling of up to 20 cells for 24 h in a dynamic mode. The cells consist of an inlet compartment, a donor compartment and a receptor compartment. The size and the depth of the inlet compartment were important to avoid entrapment of air bubbles in the receptor compartment. The Kelder cells mimic blood flow beneath the skin by replacement of the permeating drug every 2 min. Hence sink condition are more easily maintained than with the static Franz diffusion cell. The performance of the cells was tested with permeation experiments using atropine as a model drug permeating through an artificial membrane (Silastic). The use of this skin model minimized the variability in permeation of atropine as compared with human skin. |
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Authors:
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I J Bosman; A L Lawant; S R Avegaart; K Ensing; R A de Zeeuw |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 14 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1997-01-07 Completed Date: 1997-01-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1015-23 Citation Subset: IM |
Affiliation:
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Groningen Institute for Drug Studies (GIDS), University Centre for Pharmacy, Department of Analytical Chemistry and Toxicology, Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Cutaneous* Atropine / metabolism Cell Membrane Permeability Diffusion Female Humans Membranes, Artificial Permeability Skin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Membranes, Artificial; 51-55-8/Atropine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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