Document Detail


Novel cyanide inhibition at cytochrome c1 of Rhodobacter capsulatus cytochrome bc1.
MedLine Citation:
PMID:  15100019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidized cytochrome c(1) in photosynthetic bacterium Rhodobacter capsulatus cytochrome bc(1) reversibly binds cyanide with surprisingly high, micromolar affinity. The binding dramatically lowers the redox midpoint potential of heme c(1) and inhibits steady-state turnover activity of the enzyme. As cytochrome c(1), an auxiliary redox center of the high-potential chain of cytochrome bc(1), does not interact directly with the catalytic quinone/quinol binding sites Q(o) and Q(i), cyanide introduces a novel, Q-site independent locus of inhibition. This is the first report of a reversible inhibitor that manipulates the energetics and electron transfers of the high-potential redox chain of cytochrome bc(1), while maintaining quinone substrate catalytic sites in an intact form.
Authors:
Artur Osyczka; Christopher C Moser; P Leslie Dutton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1655     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-21     Completed Date:  2004-06-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-6     Citation Subset:  IM    
Affiliation:
The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, 1004 Stellar-Chance Building, 422 Curie Boulevard, Philadelphia, PA 19104, USA. osyczkaa@mail.med.upenn.edu
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cyanides / metabolism,  pharmacology*
Cytochromes c1 / antagonists & inhibitors*,  chemistry,  metabolism
Electron Transport
Electron Transport Complex III / chemistry,  metabolism
Energy Metabolism
Oxidation-Reduction
Rhodobacter capsulatus / metabolism
Spectrophotometry
Grant Support
ID/Acronym/Agency:
GM-27309/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cyanides; 9035-42-1/Cytochromes c1; EC 1.10.2.2/Electron Transport Complex III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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