Document Detail

Novel compounds produced by Streptomyces lydicus NRRL 2433 engineered mutants altered in the biosynthesis of streptolydigin.
MedLine Citation:
PMID:  22569159     Owner:  NLM     Status:  Publisher    
Streptolydigin is a tetramic acid antibiotic produced by Streptomyces lydicus NRRL 2433 and involving a hybrid polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) system in its biosynthesis. The streptolydigin amino-acid precursor, 3-methylaspartate, has been proposed to be condensed to the polyketide portion of the molecule by a NRPS composed by three enzymes (SlgN1, SlgN2 and SlgL). On the other hand, biosynthesis of the polyketide moiety involves the participation of cytochrome P450 SlgO2 for the correct cyclization of the characteristic bicyclic ketal. Independent disruption of slgN1, slgN2, slgL or slgO2 resulted in S. lydicus mutants unable to produce the antibiotic thus confirming the involvement of these genes in the biosynthesis of the antibiotic. These mutants did not accumulate any streptolydigin biosynthesis intermediate or shunt product derived from early polyketides released from the PKS. However, they produced three novel compounds identified as 4-(2-carboxy-propylamino)-3-chloro-benzoic acid, 4-(2-carboxy-propylamino)-3-hydroxy-benzoic acid and 4-(2-carboxy-propylamino)-benzoic acid, which were designated as christolane A, christolane B and christolane C, respectively. These compounds have been shown to exert some antibiotic activity.The Journal of Antibiotics advance online publication, 9 May 2012; doi:10.1038/ja.2012.37.
Cristina Gómez; Carlos Olano; Martina Palomino-Schätzlein; Antonio Pineda-Lucena; Rodrigo J Carbajo; Alfredo F Braña; Carmen Méndez; José A Salas
Related Documents :
24684439 - Global metabolite profiling reveals transformation pathways and novel metabolomic respo...
10514329 - Tanikolide, a toxic and antifungal lactone from the marine cyanobacterium lyngbya majus...
23716719 - Amino acid deprivation inhibits torc1 through a gtpase-activating protein complex for t...
956039 - Isolation of a new antibiotic 333-25, related to antibiotic em 49. (studies on antibiot...
24548779 - Production of jatropha biodiesel fuel over sulfonic acid-based solid acids.
24275979 - The action of exogenous gibberellic acid on protein and mrna in germinating castor bean...
17030019 - Long-chain multiple methyl-branched fatty acid-containing lipids of mycobacterium tuber...
5485079 - Metabolic fate of cysteine and methionine in rumen digesta.
9762349 - Regulation of secretory type-ii phospholipase a2 and of lysophosphatidic acid synthesis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-09
Journal Detail:
Title:  The Journal of antibiotics     Volume:  -     ISSN:  0021-8820     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Histamine modulates microglia function.
Next Document:  Time-series analysis of the relationship of antimicrobial use and hand hygiene promotion with the in...