Document Detail

Novel cell-surface peptides specific to human oral squamous cell carcinoma using an E. coli peptide display library.
MedLine Citation:
PMID:  17342316     Owner:  NLM     Status:  MEDLINE    
We attempted to find a specific antigen of oral squamous cell carcinoma (SCC) cells that could be safely applied to gene therapy in the conservative clinical treatment of oral cancer. We performed subtraction using normal human keratinocyte cells, followed by selection using four oral SCC cell lines. We isolated three clones from poorly differentiated SCC cells and four from well-differentiated SCC cells. These seven clones adsorbed to the oral SCC cells at rates 10-100 times those of normal human keratinocyte cells. The three clones from the poorly differentiated SCC cells showed the same peptide sequence (LAPRTHP). Of the four clones from the well-differentiated SCC cells, three showed the same peptide sequence (FGTLPGT) and the fourth showed a different one (VTPNSTP). Each peptide sequence may recognize the material that exists specifically on the oral SCC cell cortex. We can expect applications not only for tumor-targeting treatment using a gene therapy virus vector but also for diagnosis using, as a tumor marker, the peculiar SCC surface material that these peptides recognize.
Noriko Kawai; Junichi Asaumi; Jun Murakami; Toru Wakasa; Masahiro Kuroda; Miki Hisatomi; Teruhisa Unetsubo; Yuu Maki; Hidenobu Matsuzaki; Yoshinonu Yanagi; Hironobu Konouchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  17     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-07     Completed Date:  2007-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  787-91     Citation Subset:  IM    
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8525, Japan.
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MeSH Terms
Antigens, Neoplasm / genetics,  isolation & purification*
Carcinoma, Squamous Cell / chemistry*,  therapy
Cell Membrane / chemistry
Escherichia coli / genetics
Gene Therapy
Mouth Neoplasms / chemistry*,  therapy
Peptide Library*
Peptides / genetics,  isolation & purification*
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Peptide Library; 0/Peptides

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