Document Detail

Novel cell seeding system into a porous scaffold using a modified low-pressure method to enhance cell seeding efficiency and bone formation.
MedLine Citation:
PMID:  18019362     Owner:  NLM     Status:  MEDLINE    
The efficient seeding of cells into porous scaffolds is important in bone tissue engineering techniques. To enhance efficiency, we modified the previously reported cell seeding techniques using low-pressure conditions. In this study, the effects of low pressure on bone marrow-derived stromal cells (BMSCs) of rats and the usefulness of the modified technique were assessed. There was no significant difference found in the proliferative and osteogenic capabilities among various low-pressure (50-760 mmHg, 1-10 min) conditions. To analyze the efficacies of the cell seeding techniques, BMSCs suspended in the plasma of rats were seeded into porous beta-tricalcium phosphate (beta-TCP) blocks by the following three procedures: 1) spontaneous penetration of cell suspension under atmospheric pressure (SP); 2) spontaneous penetration and subsequent low pressure treatment (SPSL), the conventional technique; and 3) spontaneous penetration under low pressure conditions (SPUL), the modified technique. Subsequently, these BMSCs/beta-TCP composites were used for the analysis of cell seeding efficiency or in vivo bone formation capability. Both the number of BMSCs seeded into beta-TCP blocks and the amount of bone formation of the SPUL group were significantly higher than those of the other groups. The SPUL method with a simple technique permits high cell seeding efficiency and is useful for bone tissue engineering using BMSCs and porous scaffolds.
Ichiro Torigoe; Shinichi Sotome; Akio Tsuchiya; Toshitaka Yoshii; Makoto Takahashi; Shigenori Kawabata; Kenichi Shinomiya
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  16     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  729-39     Citation Subset:  IM    
Section of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
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MeSH Terms
Biocompatible Materials / chemistry,  metabolism
Bone Marrow Cells* / cytology,  physiology
Bone and Bones* / cytology,  physiology
Calcium Phosphates / chemistry,  metabolism
Cell Culture Techniques* / instrumentation
Cells, Cultured
Materials Testing
Mesenchymal Stem Cells* / cytology,  physiology
Osteogenesis / physiology*
Rats, Inbred F344
Tissue Engineering* / instrumentation,  methods
Reg. No./Substance:
0/Biocompatible Materials; 0/Calcium Phosphates; 0/beta-tricalcium phosphate

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