| Novel cell lines derived by long-term culture of primary uveal melanomas. | |
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MedLine Citation:
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PMID: 19212147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Uveal melanomas with or without monosomy 3 have different metastatic potentials. Therefore, it would be of great experimental importance to obtain cell lines of both groups - established and characterized under the same conditions. METHODS: Long-term culture of biopsies derived from untreated primary uveal melanomas was performed, and the status of chromosome 3 in the tumour was determined. Characterization by immunocytochemistry and in vitro assays for cell migration, vasculogenic cord formation and proliferation were performed. RESULTS: Establishment of cell lines succeeded in 6 out of 128 uveal melanomas. No histopathological feature was predictive for cultivation success. Two out of 4 cell lines derived from tumours with monosomy 3 were invasive in vitro. Both cell lines with disomy 3 showed higher proliferation levels (p = 0.002). CONCLUSIONS: The in vitro tendency for invasion did not correlate with the proliferative behaviour of uveal melanoma cell lines, but was partly associated with monosomy of chromosome 3. |
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Authors:
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Gordon Nareyeck; Michael Zeschnigk; Norbert Bornfeld; Gerasimos Anastassiou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-12 |
Journal Detail:
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Title: Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde Volume: 223 ISSN: 1423-0267 ISO Abbreviation: Ophthalmologica Publication Date: 2009 |
Date Detail:
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Created Date: 2009-05-14 Completed Date: 2009-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0054655 Medline TA: Ophthalmologica Country: Switzerland |
Other Details:
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Languages: eng Pagination: 196-201 Citation Subset: IM |
Copyright Information:
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Copyright 2009 S. Karger AG, Basel. |
Affiliation:
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Department of Ophthalmology, Universitätsklinikum Essen, Essen, Germany. gordon.nareyeck@uni-due.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biopsy Cell Culture Techniques / methods Cell Division Cell Line, Tumor Cell Movement Cell Survival Chromosomes, Human, Pair 3 Humans Melanoma / genetics, secondary* Monosomy Neoplasm Metastasis Uvea / pathology* Uveal Neoplasms / genetics, pathology* |
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