Document Detail


Novel cell lines derived by long-term culture of primary uveal melanomas.
MedLine Citation:
PMID:  19212147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Uveal melanomas with or without monosomy 3 have different metastatic potentials. Therefore, it would be of great experimental importance to obtain cell lines of both groups - established and characterized under the same conditions. METHODS: Long-term culture of biopsies derived from untreated primary uveal melanomas was performed, and the status of chromosome 3 in the tumour was determined. Characterization by immunocytochemistry and in vitro assays for cell migration, vasculogenic cord formation and proliferation were performed. RESULTS: Establishment of cell lines succeeded in 6 out of 128 uveal melanomas. No histopathological feature was predictive for cultivation success. Two out of 4 cell lines derived from tumours with monosomy 3 were invasive in vitro. Both cell lines with disomy 3 showed higher proliferation levels (p = 0.002). CONCLUSIONS: The in vitro tendency for invasion did not correlate with the proliferative behaviour of uveal melanoma cell lines, but was partly associated with monosomy of chromosome 3.
Authors:
Gordon Nareyeck; Michael Zeschnigk; Norbert Bornfeld; Gerasimos Anastassiou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-12
Journal Detail:
Title:  Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde     Volume:  223     ISSN:  1423-0267     ISO Abbreviation:  Ophthalmologica     Publication Date:  2009  
Date Detail:
Created Date:  2009-05-14     Completed Date:  2009-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0054655     Medline TA:  Ophthalmologica     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  196-201     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
Department of Ophthalmology, Universitätsklinikum Essen, Essen, Germany. gordon.nareyeck@uni-due.de
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MeSH Terms
Descriptor/Qualifier:
Biopsy
Cell Culture Techniques / methods
Cell Division
Cell Line, Tumor
Cell Movement
Cell Survival
Chromosomes, Human, Pair 3
Humans
Melanoma / genetics,  secondary*
Monosomy
Neoplasm Metastasis
Uvea / pathology*
Uveal Neoplasms / genetics,  pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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