| Novel cGMP liposomal vectors mediate efficient gene transfer. | |
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MedLine Citation:
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PMID: 12679804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Viral vector systems are the most commonly used gene transfer tools for clinical gene therapy. However, lipofection systems are potential alternatives because of lower immunogenicity and easier cGMP production, but in vivo stability and transduction efficacy need to be improved. Therefore, we investigated gene transduction efficiency of our novel cGMP cationic lipids, CCQ22 and CCQ32, by FACS analysis. Toxicity analysis was performed to determine the cytotoxic side effects of the novel lipids. To evaluate the stability of the compounds in the context of local delivery to patients with intraperitoneally metastatic ovarian cancer, gene transfer was also tested in the presence of malignant ascites. Our novel cGMP standard lipids mediated gene transfer rates of more than 50%. However, for most cell lines cytotoxic side effects were similar to our reference lipofection system. In general, ascites had no major influence on gene transduction rates with the novel lipids. Our results suggest that CCQs may compare favorably with commercially available lipofection systems. These promising results facilitate further analysis of the compounds. |
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Authors:
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Gernot Röder; Oliver Keil; Hans-Bernd Prisack; Gerd Bauerschmitz; Bettina Hanstein; Caroline Nestle-Krämling; Akseli Hemminki; Hans-Georg Bender; Dieter Niederacher; Peter Dall |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer gene therapy Volume: 10 ISSN: 0929-1903 ISO Abbreviation: Cancer Gene Ther. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-04-07 Completed Date: 2003-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9432230 Medline TA: Cancer Gene Ther Country: England |
Other Details:
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Languages: eng Pagination: 312-7 Citation Subset: IM |
Affiliation:
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MolGenLab, Department of Obstetrics/Gynecology, Duesseldorf University Medical Center, Moorenstrasse 5, 40225 Duesseldorf, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascites
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metabolism Breast Neoplasms / metabolism*, therapy Cell Line, Tumor Cholesterol Esters Female Genetic Vectors* / toxicity Humans Lipids / chemistry Liposomes* / chemistry, toxicity Ovarian Neoplasms / metabolism*, therapy Phosphatidylethanolamines Plasmids / genetics Transduction, Genetic / methods* |
| Chemical | |
Reg. No./Substance:
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0/1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; 0/Cholesterol Esters; 0/FuGene; 0/Lipids; 0/Liposomes; 0/Phosphatidylethanolamines; 76391-83-8/1,2-dielaidoylphosphatidylethanolamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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