Document Detail

Novel biodegradable cholesterol-modified polyrotaxane hydrogels for cartilage regeneration.
MedLine Citation:
PMID:  15648570     Owner:  NLM     Status:  MEDLINE    
Cholesterol was introduced to a hydrolyzable polyrotaxane (PRx), not only to improve cell proliferation and glycosaminoglycan (GAG) production, but also to control the degradation rate of the hydrogels. The cholesterol was introduced to hydrolyzable PRx species by threading many alpha-cyclodextrins (alpha-CDs) on a poly(ethylene glycol) (PEG) chain having hydrolyzable ester linkages at the terminals; the PRx species were then cross-linked with other PEGs to prepare cholesterol-modified PRx hydrogels. The degree of cholesterol substitution was varied in the range of 1-25%. These hydrogels were examined to clarify the effect of cholesterol groups on mechanical properties, erosion time and chondrocyte proliferation. Highly porous biodegradable cholesterol-modified PRx hydrogels were fabricated using a combination of potassium hydrogen carbonate (as an effervescent salt) and citric acid. This fabrication process enabled the homogeneous expansion of pores within the polymer matrices, leading to well-interconnected macroporous hydrogels with a mean pore size of around 200-400 microm, ideal for high-density chondrocyte seeding. Time to complete degradation of the hydrogels was shortened by increasing the degree of substitution due to the aggregation of alpha-CDs through hydrophobic interaction of cholesterol groups. The presence of approx. 10% cholesterol improved the chondrocyte proliferation and GAG production. The modification of cholesterols to PRx is a good approach for creating new biodegradable hydrogels in terms of chondrocyte culture and controlling degradation time of the hydrogels.
Wanpen Tachaboonyakiat; Tomoyuki Furubayashi; Masakazu Katoh; Tooru Ooya; Nobuhiko Yui
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  15     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2004  
Date Detail:
Created Date:  2005-01-14     Completed Date:  2005-06-30     Revised Date:  2008-02-20    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1389-404     Citation Subset:  IM    
Innovation Plaza Ishikawa, Japan Science and Technology Agency, 2-13 Asahidai, Tatsunokuchi, Ishikawa 923-1211, Japan.
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MeSH Terms
Biocompatible Materials / chemical synthesis,  chemistry,  metabolism*
Cartilage / cytology,  metabolism*
Cell Proliferation
Cells, Cultured
Cholesterol / chemistry*
Chondrocytes / cytology,  metabolism
Cyclodextrins / chemistry*
Glycosaminoglycans / biosynthesis,  metabolism
Hydrogels / chemical synthesis*,  chemistry,  metabolism*
Microscopy, Electron, Scanning
Poloxamer / chemistry*
Rotaxanes / chemistry*
Water / chemistry
Reg. No./Substance:
0/Biocompatible Materials; 0/Cyclodextrins; 0/Glycosaminoglycans; 0/Hydrogels; 0/Rotaxanes; 0/glucosaminoglycans; 0/polyrotaxane; 106392-12-5/Poloxamer; 57-88-5/Cholesterol; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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