| Novel approaches with track segment alpha particles and cell co-cultures in studies of bystander effects. | |
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MedLine Citation:
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PMID: 12194293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is now a significant body of data that indicate that the effects of ionising radiation may extend to more than those cells that directly suffer damage to DNA in the cell nucleus. Cells neighbouring those cells that are irradiated, or even well separated from those that are irradiated demonstrate several responses that are recorded in hit cells as a function of absorbed dose. That is, the responding non-hit cells are bystanders of hit cells. A protocol has been devised which allows for examination of one means of eliciting bystander responses, specifically, effects on non-contacting cells. Cell culture chambers are set up such that a population of cells is physically separate from the energy depositions of track segment charged particles. Absorption of energy in sub-millimetre distances in the cell culture medium ensures that one population of cells can only respond to factors generated in the irradiated medium or in another population of irradiated co-cultured cells, which may be of similar or dissimilar origin. For irradiation of medium alone, enhanced levels of micronuclei, and of delays in cell cycle progression occur in normal human fibroblasts, but not epithelial cells. This procedure allows for a defining of the factors responsible for initiating bystander effects and for determining their quantitative relevance. |
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Authors:
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C R Geard; G Jenkins-Baker; S A Marino; B Ponnaiya |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Radiation protection dosimetry Volume: 99 ISSN: 0144-8420 ISO Abbreviation: Radiat Prot Dosimetry Publication Date: 2002 |
Date Detail:
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Created Date: 2002-08-26 Completed Date: 2003-03-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8109958 Medline TA: Radiat Prot Dosimetry Country: England |
Other Details:
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Languages: eng Pagination: 233-6 Citation Subset: IM |
Affiliation:
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Radiological Research Accelerator Facility, Center for Radiological Research, College of Physicians and Surgeons of Columbia University, 136 South Broadway, PO Box 21, Irvington, NY 10533, USA. crg4@columbia.edn |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alpha Particles* Animals Bystander Effect / radiation effects* Cell Line Coculture Techniques* DNA / radiation effects* Dose-Response Relationship, Radiation Particle Accelerators |
| Grant Support | |
ID/Acronym/Agency:
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CA-49062/CA/NCI NIH HHS; CA-75061/CA/NCI NIH HHS; RR-11623/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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