| Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation. | |
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MedLine Citation:
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PMID: 18812228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neurosporaxanthin, beta-apo-4'-carotenoic acid (C35), represents the end-product of the carotenoid pathway in Neurospora crassa. It is supposed to be synthesized in three steps catalyzed by sequential AL-2, CAO-2 and YLO-1 activities: (i) cyclization of 3,4-didehydrolycopene (C40); (ii) cleavage of torulene into beta-apo-4'-carotenal (C35); and finally (iii) oxidation of beta-apo-4'-carotenal. However, analyses of the ylo-1 mutant revealed the accumulation of intermediates other than beta-apo-4'-carotenal. Here, we generated a 3,4-didehydrolycopene accumulating Escherichia coli strain and showed that CAO-2 cleaves this acyclic carotene in vivo and in vitro yielding apo-4'-lycopenal. The apocarotenoids accumulated in the ylo-1 mutant were then identified as apo-4'-lycopenal and apo-4'-lycopenol, pointing to the former as the YLO-1 substrate and indicating that cyclization is the last step in neurosporaxanthin biosynthesis. This was further substantiated by analyses of a cyclase-deficient al-2 mutant, revealing the accumulation of apo-4'-lycopenoic acid. The three acyclic apocarotenoids presented here have not been found naturally before. |
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Authors:
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Alejandro F Estrada; Dominic Maier; Daniel Scherzinger; Javier Avalos; Salim Al-Babili |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-10 |
Journal Detail:
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Title: Fungal genetics and biology : FG & B Volume: 45 ISSN: 1096-0937 ISO Abbreviation: Fungal Genet. Biol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-10 Completed Date: 2008-11-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607601 Medline TA: Fungal Genet Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1497-505 Citation Subset: IM |
Affiliation:
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Department of Genetics, Faculty of Biology, University of Seville, E-41012 Seville, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosynthetic Pathways* Carotenoids / biosynthesis*, genetics Fungal Proteins / genetics, metabolism Gene Expression Regulation, Fungal Lactones / metabolism Mutation* Neurospora crassa / enzymology, genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Lactones; 0/lycoperdic acid; 0/neurosporaxanthin; 36-88-4/Carotenoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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