Document Detail

Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression.
MedLine Citation:
PMID:  11500571     Owner:  NLM     Status:  MEDLINE    
Two cDNA clones (NtmybAS1 and NtmybAS2) encoding MYB-related proteins with strong sequence similarity to petunia (Petunia hybrida) PhMYB3 were isolated from a tobacco (Nicotiana tabacum cv Samsun) pollen cDNA library. Northern blot and in situ hybridization revealed that NtmybAS transcripts are specifically expressed in both sporophytic and gametophytic tissues of the anther including tapetum, stomium, vascular tissue, and developing pollen. Random binding site selection assays revealed that NtMYBAS1 bound to DNA sequences closely resembling consensus MYB binding sites MBSI and MBSIIG, with a higher affinity for MBSI. Transient expression analyses of the N-terminal MYB domain demonstrated the presence of functional nuclear localization signals, and full-length NtMYBAS1 was able to activate two different phenylalanine ammonia-lyase promoters (PALA and gPAL1) in tobacco leaf protoplasts. Similar analysis of truncated NtmybAS1 cDNAs identified an essential, C-terminal trans-activation domain. Further in situ hybridization analyses demonstrated strict co-expression of NtmybAS and gPAL1 in the tapetum and stomium. Despite abundant expression of NtmybAS transcripts in mature pollen, gPAL1 transcripts were not detectable in pollen. Our data demonstrate that NtMYBAS1 is a functional anther-specific transcription factor, which is likely to be a positive regulator of gPAL1 expression and phenylpropanoid synthesis in sporophytic, but not in gametophytic, tissues of the anther.
S Yang; J P Sweetman; S Amirsadeghi; M Barghchi; A K Huttly; W I Chung; D Twell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  126     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-13     Completed Date:  2001-10-11     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1738-53     Citation Subset:  IM    
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Cloning, Molecular
DNA, Complementary
DNA, Plant
Molecular Sequence Data
Multigene Family
Phenylalanine Ammonia-Lyase / genetics*,  metabolism
Phenylpropionates / metabolism
Plant Proteins*
Plant Stems
Plants, Toxic*
Pollen / genetics
Sequence Homology, Amino Acid
Tobacco / enzymology,  genetics*
Transcription Factors / genetics*,  metabolism
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/NtMYBAS1 protein, Nicotiana tabacum; 0/NtMYBAS2 protein, Nicotiana tabacum; 0/Phenylpropionates; 0/Plant Proteins; 0/Transcription Factors; EC Ammonia-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chemical composition of the Prunus laurocerasus leaf surface. Dynamic changes of the epicuticular wa...
Next Document:  30 years later: On the problem of the relation between structure and function in the brain from a co...