| Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins. | |
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MedLine Citation:
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PMID: 22214319 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lysozyme and insulin were encapsulated in alginate gel microspheres using impinging aerosols method. High loadings of around 50% weight/dry microspheres weight were obtained with encapsulation efficiencies of at least 48%. Environmental scanning electron microscopy revealed smooth spherical hydrated microspheres (30-60 µm) in diameter. No lysozyme or insulin release was measured in simulated gastric fluid (HCl, pH 1.2, 37°C). Total insulin release occurred in simulated intestinal fluid (SIF; phosphate buffer saline, pH 7.4, 37°C) in 8 h following 2 h incubation in SGF and was found to retain 75% activity using the ARCHITECT® assay. Lysozyme was released completely in SIF in 10 h following 2 h incubation in SGF and was found to exhibit at least 80% bioactivity using the Micrococcus lysodeikticus assay. The absence of protein release in HCl and the retention of high levels of biological activity demonstrate the potential of alginate gel microspheres, for improving oral delivery of biopharmaceuticals. |
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Authors:
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Dewi Melani Hariyadi; Yiwei Wang; Sharon Chien-Yu Lin; Thor Bostrom; Bhesh Bhandari; Allan G A Coombes |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-4 |
Journal Detail:
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Title: Journal of microencapsulation Volume: - ISSN: 1464-5246 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8500513 Medline TA: J Microencapsul Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Pharmacy Australia Centre of Excellence, The University of Queensland , Brisbane, QLD 4102 , Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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