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Novel X-Linked Genes Revealed by qPCR in the Green Anole, Anolis carolinensis.
MedLine Citation:
PMID:  25172916     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The green anole, Anolis carolinensis (ACA), is the model reptile for a vast array of biological disciplines. It was the first non-avian reptile to have its genome fully sequenced. During the genome project, the XX/XY system of sex chromosomes homologous to chicken chromosome 15 (GGA15) was revealed and 106 X-linked genes were identified. We selected 38 genes located on eight scaffolds in A. carolinensis and having orthologs located on GGA15, then tested their linkage to A. carolinensis X chromosome using comparative quantitative fluorescent real-time PCR applied to male and female genomic DNA.All tested genes appeared to be X-specific and not present on the Y chromosome. Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in A. carolinensis, from 106 to 250. While demonstrating that the gene content of chromosome X in A. carolinensis and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages. The presence of many ACA X-specific genes localized to distinct contigs indicates that the ACA Y chromosome should be highly degenerated, having lost a large amount of its original gene content during evolution. The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics.
Authors:
Michail Rovatsos; Marie Altmanová; Martina Johnson Pokorná; Lukáš Kratochvíl
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-28
Journal Detail:
Title:  G3 (Bethesda, Md.)     Volume:  -     ISSN:  2160-1836     ISO Abbreviation:  G3 (Bethesda)     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101566598     Medline TA:  G3 (Bethesda)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Author et al.
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