Document Detail


A novel transgenic mouse for gene-targeting within cells that express corticotropin-releasing factor.
MedLine Citation:
PMID:  20303068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Corticotropin-releasing factor (CRF) orchestrates the mammalian endocrine, autonomic, and behavioral stress response and has been implicated in the pathophysiology of illnesses ranging from irritable bowel syndrome to mood and anxiety disorders. CRF is produced and released from a variety of cell types, making it difficult to distinguish the specific role of CRF from other neurotransmitters with which it colocalizes. To clarify the basic biology of the CRF neuron, we must be able to manipulate selectively CRFergic cells. Here we describe a novel transgenic mouse using 3.0 kb of the CRF promoter to drive expression of Cre-recombinase (CRFp3.0Cre). Crossing CRFp3.0Cre with a fluorescent reporter strain results in Cre-dependent green fluorescent protein expression within CRF-producing cells. Thus, CRF cells can be identified for single-cell polymerase chain reaction and electrophysiological procedures. Furthermore, the CRFp3.0Cre transgenic can be combined with other available mouse strains containing a "floxed" gene of interest to allow unparalleled detailed analysis of the CRF system.
Authors:
Elizabeth I Martin; Kerry J Ressler; Aaron M Jasnow; Joanna Dabrowska; Rimi Hazra; Donald G Rainnie; Charles B Nemeroff; Michael J Owens
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-29
Journal Detail:
Title:  Biological psychiatry     Volume:  67     ISSN:  1873-2402     ISO Abbreviation:  Biol. Psychiatry     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-07     Completed Date:  2010-10-25     Revised Date:  2013-06-21    
Medline Journal Info:
Nlm Unique ID:  0213264     Medline TA:  Biol Psychiatry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1212-6     Citation Subset:  IM    
Affiliation:
Peptide Biology Laboratory, The Salk Institute, La Jolla, CA, USA. emartin@salk.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism,  physiology
Corticotropin-Releasing Hormone / genetics*,  metabolism
Gene Targeting / methods*
Integrases / genetics,  metabolism
Mice
Mice, Transgenic*
Neurons / metabolism,  physiology
Grant Support
ID/Acronym/Agency:
DA019624/DA/NIDA NIH HHS; F31 MH079667-01A1/MH/NIMH NIH HHS; MH-39415/MH/NIMH NIH HHS; MH-42088/MH/NIMH NIH HHS; MH-541380/MH/NIMH NIH HHS; MH-58922/MH/NIMH NIH HHS; MH-69056/MH/NIMH NIH HHS; MH-77083/MH/NIMH NIH HHS; MH069852/MH/NIMH NIH HHS; MH072908/MH/NIMH NIH HHS; MH079667A/MH/NIMH NIH HHS; R01 MH072908/MH/NIMH NIH HHS; RR-00039/RR/NCRR NIH HHS; UL1 RR025008/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
9015-71-8/Corticotropin-Releasing Hormone; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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