| A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids. | |
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MedLine Citation:
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PMID: 19818421 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O. |
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Authors:
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Yen Kuang Li; Den-Kai Yang; Yun-Chu Chen; Hung-Ju Su; Jui-Chuang Wu; Yui Whei Chen-Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-07 |
Journal Detail:
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Title: Acta biomaterialia Volume: 6 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 1462-70 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Chemistry, Chung Yuan Christian University, 200 Chung-Pei Road, Chung-Li, Taoyuan County 32023, Taiwan, ROC. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA Probes
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chemistry* Gels / chemistry* Humans Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Nitrogen / chemistry Nucleic Acid Conformation Nucleotides / analysis*, genetics, metabolism Oligonucleotide Array Sequence Analysis / methods* Organosilicon Compounds / chemistry* Polymers / chemistry* Silicon Dioxide / chemistry Spectroscopy, Fourier Transform Infrared Temperature |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Gels; 0/Nucleotides; 0/Organosilicon Compounds; 0/Polymers; 7631-86-9/Silicon Dioxide; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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