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Novel Route of Tannic Acid Biotransformation and Their Effect on Major Biopolymer Synthesis in Azotobacter sp. SSB81.
MedLine Citation:
PMID:  23035941     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: To examine tannic acid (TA) utilization capacity by nitrogen fixing bacteria, Azotobacter sp. SSB81 and identify the intermediate products during biotransformation. Another aim of this work is to investigate the effects of TA on major biopolymers (EPS and PHB) synthesis. METHODS AND RESULTS: Tannic acid utilization and tolerance capacity of the strain was determined according to CLSI method. Intermediate products were identified using HPLC, LC-MS/MS and (1) HNMR analysis. Intermediates were quantified by multiple reactions monitoring in LC-MS/MS. The strain was able to tolerate a high level of TA and utilized through enzymatic system. Growth of Azotobacter in TA supplemented medium was characterized by an extended lag phase and decreased growth rate. Presence of TA catalytic enzymes as tannase, plyphenol oxidase and phenol decarboxylase were confirmed in cell lysate using their specific substrates. Polyphenol oxidase activity was more prominent in TA supplemented mineral medium after 48h of growth when gallic to ellagic acid reversible reaction was remarkable. Phase contrast and scanning electron microscopic analysis revealed elongated and irregular size of Azotobacter cells in response to TA. (1) H NMR analysis indicated that tannic acid was transformed into gallic acid, ellagic acid and pyrogallol. Biopolymer (EPS and PHB) production was decreased several folds in the presence of TA compared to cells grown in only glucose medium. CONCLUSIONS: This is the first evidence on biotransformation of tannic acid by Azotobacter and also elevated level of ellagic acid production from gallotaninns. Azotobacter has developed the mechanism to utilize TA for their carbon and energy source. SIGNIFICANCE AND IMPACT OF THE STUDY: The widespread occurrence and exploitation of Azotobacter sp. Strain SSB81 in agricultural and forest soil have an additional advantage to utilize the soil accumulated TA and detoxifies the allelopathic effect of constant accumulated TA in soil. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Samiran S Gauri; Santi M Mandal; Sanghamitra Atta; Satyahari Dey; Bikas R Pati
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Department of Microbiology, Vidyasagar University, Midnapore, 721102, W B, India; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302.
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