| A Novel Platform for the Detection of Staphylococcus aureus Enterotoxin B in Foods. | |
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MedLine Citation:
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PMID: 23241982 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Staphylococcal contamination of food products and staphylococcal foodborne illnesses continue to be a problem worldwide. Screening food for the presence of Staphylococcal aureus and/or enterotoxins using traditional methods is laborious. Reliable and rapid multiplex detection methods from a single food extract or culture supernatant would simplify testing. A fluorescence-based cytometric bead array was developed for the detection of staphylococcal enterotoxin B (SEB) using magnetic microspheres coupled with either an engineered, enterotoxin specific, Vβ domain of the T-cell receptor (Vβ TCR) or polyclonal antibodies. The binding affinity of the Vβ TCR SEB has been shown to be in the picomolar range, comparable to the best monoclonal antibodies. The coupled beads were validated with purified enterotoxins and tested in a variety of food matrices spiked with enterotoxins. The Vβ TCR or antibody was shown to specifically bind SEB in four different food matrices including milk, mashed potatoes, vanilla pudding and cooked chicken. The use of traditional polyclonal antibodies and Vβ TCR provides a redundant system that ensures accurate identification of the enterotoxin and the use of labeled microspheres permits simultaneous testing of multiple enterotoxins from a single sample. |
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Authors:
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Sandra M Tallent; Jeffrey A Degrasse; Ningyan Wang; Daiva M Mattis; David M Kranz |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-14 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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U.S. FDA Center for Food Safety and Applied Nutrition, College Park, MD 20740. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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