Document Detail


Novel platform for the detection of Staphylococcus aureus enterotoxin B in foods.
MedLine Citation:
PMID:  23241982     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcal contamination of food products and staphylococcal food-borne illnesses continue to be a problem worldwide. Screening of food for the presence of Staphylococcus aureus and/or enterotoxins using traditional methods is laborious. Reliable and rapid multiplex detection methods from a single food extract or culture supernatant would simplify testing. A fluorescence-based cytometric bead array was developed for the detection of staphylococcal enterotoxin B (SEB), using magnetic microspheres coupled with either an engineered, enterotoxin-specific Vβ domain of the T-cell receptor (Vβ-TCR) or polyclonal antibodies. The binding affinity of the Vβ-TCR for SEB has been shown to be in the picomolar range, comparable to the best monoclonal antibodies. The coupled beads were validated with purified enterotoxins and tested in a variety of food matrices spiked with enterotoxins. The Vβ-TCR or antibody was shown to specifically bind SEB in four different food matrices, including milk, mashed potatoes, vanilla pudding, and cooked chicken. The use of traditional polyclonal antibodies and Vβ-TCR provides a redundant system that ensures accurate identification of the enterotoxin, and the use of labeled microspheres permits simultaneous testing of multiple enterotoxins from a single sample.
Authors:
Sandra M Tallent; Jeffrey A Degrasse; Ningyan Wang; Daiva M Mattis; David M Kranz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Validation Studies     Date:  2012-12-14
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  79     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-14     Completed Date:  2013-07-26     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1422-7     Citation Subset:  IM    
Affiliation:
U.S. FDA Center for Food Safety and Applied Nutrition, College Park, MD, USA. Sandra.Tallent@fda.hhs.gov
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antitoxins / immunology
Enterotoxins / analysis*
Food Analysis / methods*
Immunomagnetic Separation / methods*
Receptors, Antigen, T-Cell / genetics,  immunology
Grant Support
ID/Acronym/Agency:
F30 HL096352/HL/NHLBI NIH HHS; HHSN272200700055C//PHS HHS; NRS100//PHS HHS; NRS109//PHS HHS; NRS111//PHS HHS; NRS113//PHS HHS; NRS114//PHS HHS; NRS120//PHS HHS; NRS121//PHS HHS; NRS166//PHS HHS; NRS185//PHS HHS; NRS193//PHS HHS; NRS194//PHS HHS; NRS387//PHS HHS; NRS483//PHS HHS; U54 AI57153/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antitoxins; 0/Enterotoxins; 0/Receptors, Antigen, T-Cell; 39424-53-8/enterotoxin B, staphylococcal
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Efficient plant biomass degradation by thermophilic fungus Myceliophthora heterothallica.
Next Document:  Methanogens and methanogenesis in the rumens and ceca of lambs fed two different high-grain-content ...