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Novel Photosensory Two-Component System (PixA-NixB-NixC) Involved in the Regulation of Positive and Negative Phototaxis of Cyanobacterium Synechocystis sp. PCC 6803.
MedLine Citation:
PMID:  22065076     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Two wild type substrains of a motile cyanobacterium Synechocystis sp. PCC 6803 show positive phototaxis toward a light source (PCC-P) and negative phototaxis away from light (PCC-N). In this study, we found that a novel two-component system of photoresponse is involved in the phototactic regulation. Inactivation of slr1212 (pixA), which encodes a photoreceptor histidine kinase, reverted the positive phototaxis of PCC-P to the negative phototaxis and inactivation of the downstream slr1213 (nixB) and slr1214 (nixC), which encode AraC-like transcription factor-type and PatA-type response regulators, respectively, reverted the negative phototaxis of PCC-N to the positive phototaxis. Opposite effects of pixA and nixBC disruption implies unexpected signal transduction pathway in the switching of positive and negative phototaxis. The blue/green-type cyanobacteriochrome GAF domain of PixA was expressed in Synechocystis and phycocyanobilin-producing Escherichia coli. The holoprotein covalently bound a chromophore phycoviolobilin and showed reversible photoconversion between violet-(Pv, λ(peak) = 396 nm) and green-absorbing (Pg, λ(peak) = 533 nm) forms, although the protein from E. coli partially bound a precursor phycocyanobilin. These results were discussed with regard to an idea that PixA serves as a violet light receptor for switching of positive and negative phototaxis by transcriptional and functional regulation.
Authors:
Rei Narikawa; Fumiko Suzuki; Shizue Yoshihara; Sho-Ichi Higashi; Masakatsu Watanabe; Masahiko Ikeuchi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-7
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan; Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku Sakai, Osaka 599-8531, Japan, Spectrography and Bioimaging Facility, NIBB Core Research Facilities, National Institute for Basic Biology, Okazaki, Aichi, Japan, The Graduate School for the Creation of New Photonics Industries, Kurematsu-cho 1955-1, Nishi-ku, Hamamatsu, Shizuoka 431-1202, Japan.
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