| Novel Photosensory Two-Component System (PixA-NixB-NixC) Involved in the Regulation of Positive and Negative Phototaxis of Cyanobacterium Synechocystis sp. PCC 6803. | |
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MedLine Citation:
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PMID: 22065076 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Two wild type substrains of a motile cyanobacterium Synechocystis sp. PCC 6803 show positive phototaxis toward a light source (PCC-P) and negative phototaxis away from light (PCC-N). In this study, we found that a novel two-component system of photoresponse is involved in the phototactic regulation. Inactivation of slr1212 (pixA), which encodes a photoreceptor histidine kinase, reverted the positive phototaxis of PCC-P to the negative phototaxis and inactivation of the downstream slr1213 (nixB) and slr1214 (nixC), which encode AraC-like transcription factor-type and PatA-type response regulators, respectively, reverted the negative phototaxis of PCC-N to the positive phototaxis. Opposite effects of pixA and nixBC disruption implies unexpected signal transduction pathway in the switching of positive and negative phototaxis. The blue/green-type cyanobacteriochrome GAF domain of PixA was expressed in Synechocystis and phycocyanobilin-producing Escherichia coli. The holoprotein covalently bound a chromophore phycoviolobilin and showed reversible photoconversion between violet-(Pv, λ(peak) = 396 nm) and green-absorbing (Pg, λ(peak) = 533 nm) forms, although the protein from E. coli partially bound a precursor phycocyanobilin. These results were discussed with regard to an idea that PixA serves as a violet light receptor for switching of positive and negative phototaxis by transcriptional and functional regulation. |
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Authors:
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Rei Narikawa; Fumiko Suzuki; Shizue Yoshihara; Sho-Ichi Higashi; Masakatsu Watanabe; Masahiko Ikeuchi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-7 |
Journal Detail:
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Title: Plant & cell physiology Volume: - ISSN: 1471-9053 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan; Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku Sakai, Osaka 599-8531, Japan, Spectrography and Bioimaging Facility, NIBB Core Research Facilities, National Institute for Basic Biology, Okazaki, Aichi, Japan, The Graduate School for the Creation of New Photonics Industries, Kurematsu-cho 1955-1, Nishi-ku, Hamamatsu, Shizuoka 431-1202, Japan. |
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