Document Detail


A Novel Method to Produce Immobilised Biomolecular Concentration Gradients to Study Cell Activities: Design and Modelling.
MedLine Citation:
PMID:  21544696     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
It is well known that many cell functions are activated by chemical signals with a time and space-dependent profile. To mimic these profiles in vitro, it is necessary to develop a system that is able to generate concentration gradients with a resolution similar to that perceived by cells, which is around nanomolar with a spatial resolution of a few tens of microns. Many devices capable of generating steady-state concentration gradients have been developed using continuous flow micro-fluidic techniques. However, these systems cannot reproduce the immobilised concentration gradients that are present in the extracellular matrix. For this reason, we have developed a new gradient generator to enable precise and reproducible studies on the effects of immobilised concentration gradients on cell behaviour. A well-known gradient of a desired molecule was generated on the bottom surface of a hydrogel, which was then used as a stamp to immobilise the molecule on a functionalised substrate. A concentration gradient was thus obtained using a simple silane-based chemical reaction. To validate the method, image analysis was performed on glass slides printed with fluorescein isothiocyanate (FITC)- collagen and FITC-poly-lysine concentration gradients. Preliminary cell adhesion tests were also carried out by seeding NIH-3T3 and mesencephalic cells on lab-glass slides printed with concentration profiles of collagen and poly-lysine, respectively.
Authors:
Giovanni Vozzi; Tommaso Lenzi; Francesca Montemurro; Carla Pardini; Francesca Vaglini; Arti Ahluwalia
Related Documents :
18058196 - In vitro behavior of osteoblast-like cells on plla films with a biomimetic apatite or a...
19842106 - Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism.
20561676 - Preparation of caco-2 cell sheets using plasma polymerised acrylic acid as a weak bound...
14999756 - Quantitative microcomputed tomography analysis of mineralization within three-dimension...
18997296 - Blueberry antagonism of c-2 ceramide disruption of ca2+ responses and recovery in machr...
21084696 - Hypertrophic phenotype in cardiac cell assemblies solely by structural cues and ensuing...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-5
Journal Detail:
Title:  Molecular biotechnology     Volume:  -     ISSN:  1559-0305     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Interdepartmental Research Center "E. Piaggio"-Faculty of Engineering, University of Pisa, Via Diotisalvi 2, 56126, Pisa, Italy, g.vozzi@centropiaggio.unipi.it.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Specific IgG antibody responses may be used to monitor leprosy treatment efficacy and as recurrence ...
Next Document:  Morphofunctional Evaluation of the Heart of Obese Patients Before and After Bariatric Surgery.