| A Novel Method to Produce Immobilised Biomolecular Concentration Gradients to Study Cell Activities: Design and Modelling. | |
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MedLine Citation:
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PMID: 21544696 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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It is well known that many cell functions are activated by chemical signals with a time and space-dependent profile. To mimic these profiles in vitro, it is necessary to develop a system that is able to generate concentration gradients with a resolution similar to that perceived by cells, which is around nanomolar with a spatial resolution of a few tens of microns. Many devices capable of generating steady-state concentration gradients have been developed using continuous flow micro-fluidic techniques. However, these systems cannot reproduce the immobilised concentration gradients that are present in the extracellular matrix. For this reason, we have developed a new gradient generator to enable precise and reproducible studies on the effects of immobilised concentration gradients on cell behaviour. A well-known gradient of a desired molecule was generated on the bottom surface of a hydrogel, which was then used as a stamp to immobilise the molecule on a functionalised substrate. A concentration gradient was thus obtained using a simple silane-based chemical reaction. To validate the method, image analysis was performed on glass slides printed with fluorescein isothiocyanate (FITC)- collagen and FITC-poly-lysine concentration gradients. Preliminary cell adhesion tests were also carried out by seeding NIH-3T3 and mesencephalic cells on lab-glass slides printed with concentration profiles of collagen and poly-lysine, respectively. |
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Authors:
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Giovanni Vozzi; Tommaso Lenzi; Francesca Montemurro; Carla Pardini; Francesca Vaglini; Arti Ahluwalia |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-5 |
Journal Detail:
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Title: Molecular biotechnology Volume: - ISSN: 1559-0305 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Interdepartmental Research Center "E. Piaggio"-Faculty of Engineering, University of Pisa, Via Diotisalvi 2, 56126, Pisa, Italy, g.vozzi@centropiaggio.unipi.it. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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