| A novel method for preclinical detection of PrPSc in blood. | |
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MedLine Citation:
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PMID: 20357038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we demonstrate that a moderate amount of protein misfolding cyclic amplification (PMCA) coupled to a novel surround optical fibre immunoassay (SOFIA) detection scheme can be used to detect the disease-associated form of the prion protein (PrP(Sc)) in protease-untreated plasma from preclinical and clinical scrapie sheep, and white-tailed deer with chronic wasting disease, following natural and experimental infection. PrP(Sc), resulting from a conformational change of the normal (cellular) form of prion protein (PrP(C)), is considered central to neuropathogenesis and serves as the only reliable molecular marker for prion disease diagnosis. While the highest levels of PrP(Sc) are present in the central nervous system, the development of a reasonable diagnostic assay requires the use of body fluids that characteristically contain exceedingly low levels of PrP(Sc). PrP(Sc) has been detected in the blood of sick animals by means of PMCA technology. However, repeated cycling over several days, which is necessary for PMCA of blood material, has been reported to result in decreased specificity (false positives). To generate an assay for PrP(Sc) in blood that is both highly sensitive and specific, we have utilized limited serial PMCA (sPMCA) with SOFIA. We did not find any enhancement of sPMCA with the addition of polyadenylic acid nor was it necessary to match the genotypes of the PrP(C) and PrP(Sc) sources for efficient amplification. |
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Authors:
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Richard Rubenstein; Binggong Chang; Perry Gray; Martin Piltch; Marie S Bulgin; Sharon Sorensen-Melson; Michael W Miller |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-03-31 |
Journal Detail:
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Title: The Journal of general virology Volume: 91 ISSN: 1465-2099 ISO Abbreviation: J. Gen. Virol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-18 Completed Date: 2010-07-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 1883-92 Citation Subset: IM |
Affiliation:
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Departments of Neurology and Physiology/Pharmacology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA. richard.rubenstein@downstate.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology* Blotting, Western Genotype PrPSc Proteins / blood*, genetics, immunology, isolation & purification* Protein Folding Scrapie / blood*, genetics Sheep |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/PrPSc Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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