Document Detail


A novel isolator-based system promotes viability of human embryos during laboratory processing.
MedLine Citation:
PMID:  22393356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro fertilisation (IVF) and related technologies are arguably the most challenging of all cell culture applications. The starting material is a single cell from which one aims to produce an embryo capable of establishing a pregnancy eventually leading to a live birth. Laboratory processing during IVF treatment requires open manipulations of gametes and embryos, which typically involves exposure to ambient conditions. To reduce the risk of cellular stress, we have developed a totally enclosed system of interlinked isolator-based workstations designed to maintain oocytes and embryos in a physiological environment throughout the IVF process. Comparison of clinical and laboratory data before and after the introduction of the new system revealed that significantly more embryos developed to the blastocyst stage in the enclosed isolator-based system compared with conventional open-fronted laminar flow hoods. Moreover, blastocysts produced in the isolator-based system contained significantly more cells and their development was accelerated. Consistent with this, the introduction of the enclosed system was accompanied by a significant increase in the clinical pregnancy rate and in the proportion of embryos implanting following transfer to the uterus. The data indicate that protection from ambient conditions promotes improved development of human embryos. Importantly, we found that it was entirely feasible to conduct all IVF-related procedures in the isolator-based workstations.
Authors:
Louise Hyslop; Nilendran Prathalingam; Lynne Nowak; Jeanette Fenwick; Steve Harbottle; Samantha Byerley; John Rhodes; Bruce Watson; Robin Henderson; Alison Murdoch; Mary Herbert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-29
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-06     Completed Date:  2012-07-23     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e31010     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology*
Cell Count / methods*
Embryo Transfer / methods,  standards*
Equipment Design
Female
Fertilization in Vitro*
Fetal Viability / physiology
Humans
Hydrogen-Ion Concentration
Laboratories
Mice
Observer Variation
Oocytes / cytology
Ovulation Induction
Regression Analysis
Reproductive Techniques, Assisted / instrumentation*
Sperm Injections, Intracytoplasmic / methods
Temperature
Grant Support
ID/Acronym/Agency:
096919//Wellcome Trust; G0400232//Medical Research Council; G0601157//Medical Research Council; GO400232//Medical Research Council
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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