Document Detail

A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Monolayer in Real-Time and in Situ under Confocal Laser Scanning Microscopy.
MedLine Citation:
PMID:  22382319     Owner:  NLM     Status:  In-Data-Review    
Although the cellular endocytosis or uptake research using confocal laser scanning microscopy (CLSM) and Transwell inserts was frequently reported in experimental cell research and pharmaceutical research, there is little report on cellular transport process based on the same techniques. One of main reasons is that fluorescence of most fluorescent reagents could be clearly and definitely seen under CLSM only when they are internalized into and concentrated within cells. Here, Madin-Darby Canine Kidney (MDCK) cells and Coumarin 6 labeled nanoparticles (C6-NPs) was used as models, a new system was developed to image C6-NPs transport across Transwell filter grown cell monolayer by adding free cells into the basolateral medium and making the Transwell insert semi-permeable membrane visible under CLSM. The transport process could be clearly imaged in real-time and in situ, based on the visualization of Transwell membrane indicating the relative position of cells and nanoparticles, and the free cells in the basolateral medium serving as collector and indicator for the transported nanoparticles. The method was also applied in cell migration study. We believe that the novel approach developed here will be certainly useful not only in exploration of nanoparticle cellular transport mechanism, but also in other cell biological sciences based on CLSM and Transwell.
Shanshan Zhao; Lan Yuan; Jiancheng Wang; Xuan Zhang; Zhonggui He; Qiang Zhang
Related Documents :
21259019 - Radiation-induced cell cycle arrests in ehrlich ascites carcinoma cells in vivo.
15720119 - Dynamic monitoring of cytotoxicity on microelectronic sensors.
18955299 - Differential growth suppression of human melanoma cells by tea (camellia sinensis) epic...
1764699 - Combination of the minor groove-binder u73-975 or the intercalator mitoxantrone with an...
19921789 - Alasmontamine a, a first tetrakis monoterpene indole alkaloid from tabernaemontana eleg...
7107409 - In vitro hypoxic cytotoxicity of nitroimidazoles: uptake and cell cycle phase specificity.
23299369 - Long-term, high-resolution confocal time lapse imaging of arabidopsis cotyledon epiderm...
21678129 - Borrelidin, a small molecule nitrile-containing macrolide inhibitor of threonyl-trna sy...
21822009 - Molecular and cellular evidence for the alternative lengthening of telomeres (alt) mech...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  35     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  335-45     Citation Subset:  IM    
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mechanism-Based Inhibition of Recombinant Human Cytochrome P450 3A4 by Tomato Juice Extract.
Next Document:  Possible Involvement of Transient Receptor Potential Channels in Electrophile-Induced Insulin Secret...