| Novel Dictyostelium unconventional myosin, MyoM, has a putative RhoGEF domain. | |
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MedLine Citation:
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PMID: 10828443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have cloned a novel unconventional myosin gene myoM in Dictyostelium. Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily. Following the motor domain, two calmodulin-binding IQ motifs, a putative coiled-coil region, and a Pro, Ser and Thr-rich domain, lies a combination of dbl homology and pleckstrin homology domains. These are conserved in Rho GDP/GTP exchange factors (RhoGEFs). We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyostelium cells were not affected by the myoM(-) mutation. Green fluorescent protein-tagged MyoM, however, accumulated at crown-shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis. |
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Authors:
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N Oishi; H Adachi; K Sutoh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 474 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-07-06 Completed Date: 2000-07-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 16-22 Citation Subset: IM |
Affiliation:
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Biotechnology Research Center, Teikyo University, 907 Nogawa, Miyamae-ku, Kawasaki-shi, Kanagawa 216-0001, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Binding Sites Calmodulin / metabolism Dictyostelium / chemistry*, genetics, ultrastructure Green Fluorescent Proteins Guanine Nucleotide Exchange Factors / chemistry* Humans Indicators and Reagents Luminescent Proteins Molecular Sequence Data Myosins / analysis, chemistry*, genetics Phylogeny Protozoan Proteins* Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin; 0/Guanine Nucleotide Exchange Factors; 0/Indicators and Reagents; 0/Luminescent Proteins; 0/Protozoan Proteins; 0/myoM protein, Dictyostelium discoideum; 0/rho guanine nucleotide exchange factors; 147336-22-9/Green Fluorescent Proteins; EC 3.6.4.1/Myosins |
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