Document Detail


Novel Dictyostelium unconventional myosin, MyoM, has a putative RhoGEF domain.
MedLine Citation:
PMID:  10828443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have cloned a novel unconventional myosin gene myoM in Dictyostelium. Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily. Following the motor domain, two calmodulin-binding IQ motifs, a putative coiled-coil region, and a Pro, Ser and Thr-rich domain, lies a combination of dbl homology and pleckstrin homology domains. These are conserved in Rho GDP/GTP exchange factors (RhoGEFs). We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyostelium cells were not affected by the myoM(-) mutation. Green fluorescent protein-tagged MyoM, however, accumulated at crown-shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis.
Authors:
N Oishi; H Adachi; K Sutoh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  474     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  16-22     Citation Subset:  IM    
Affiliation:
Biotechnology Research Center, Teikyo University, 907 Nogawa, Miyamae-ku, Kawasaki-shi, Kanagawa 216-0001, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Calmodulin / metabolism
Dictyostelium / chemistry*,  genetics,  ultrastructure
Green Fluorescent Proteins
Guanine Nucleotide Exchange Factors / chemistry*
Humans
Indicators and Reagents
Luminescent Proteins
Molecular Sequence Data
Myosins / analysis,  chemistry*,  genetics
Phylogeny
Protozoan Proteins*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Calmodulin; 0/Guanine Nucleotide Exchange Factors; 0/Indicators and Reagents; 0/Luminescent Proteins; 0/Protozoan Proteins; 0/myoM protein, Dictyostelium discoideum; 0/rho guanine nucleotide exchange factors; 147336-22-9/Green Fluorescent Proteins; EC 3.6.4.1/Myosins

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