| A Novel Cationic Ribonuclease with Antimicrobial Activity from Rana dybowskii. | |
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MedLine Citation:
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PMID: 21259045 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A novel ribonuclease (RNase) A superfamily gene (Rdronc) has been cloned from the frog Rana dybowskii. The deduced amino acid sequence shows that it belongs to the ribonuclease A superfamily, with the highest identity, 73%, to Rana pipiens onconase. Adaptive evolution analysis based on maximum likelihood models of codon substitution has been conducted on 10 members of the Rana RNases of subcluster B. Rapid adaptive evolution and multiple positive selection sites have been detected, which indicates that these genes may be evolving under positive selection pressure. Functional assay demonstrates that the recombinant Rdronc protein possesses antimicrobial activity against Gram-negative Escherichia coli and Pseudomonas aeruginosa and weaker antimicrobial activity against Gram-positive Staphylococcus aureus and yeast Candida albicans. Our findings support the hypothesis that ribonuclease A superfamily members may function in host defense of early-diversified vertebrates. |
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Authors:
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Fengyun Tao; Meiyin Fan; Wei Zhao; Qiang Lin; Runyu Ma |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-23 |
Journal Detail:
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Title: Biochemical genetics Volume: - ISSN: 1573-4927 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0126611 Medline TA: Biochem Genet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China, hgtfengyun@buu.edu.cn. |
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