| A novel catalytic ability of gamma-glutamylcysteine synthetase of Escherichia coli and its application in theanine production. | |
| | |
MedLine Citation:
|
PMID: 19966457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Gamma-glutamylcysteine synthetase (gammaGCS, EC 6.3.2.2) catalyzes the formation of gamma-glutamylcysteine from L-glutamic acid (Glu) and L-cysteine (Cys) in an ATP-dependent manner. While gammaGCS can use various amino acids as substrate, little is known about whether it can use non-amino acid compounds in place of Cys. We determined that gammaGCS from Escherichia coli has the ability to combine Glu and amines to form gamma-glutamylamides. The reaction rate depended on the length of the methylene chain of the amines in the following order: n-propylamine > butylamine > ethylamine >> methylamine. The optimal pH for the reaction was narrower and more alkaline than for the reaction with an amino acid. The newly found catalytic ability of gammaGCS was used in the production of theanine (gamma-glutamylethylamine). The resting cells of E. coli expressing gammaGCS, in which ATP was regenerated through glycolysis, synthesized 12.1 mM theanine (18 h) from 429 mM ethylamine. |
| | |
Authors:
|
Koichiro Miyake; Shingo Kakita |
Related Documents
:
|
8135347 - Isolation and characterization of the reaction product of 4-diazobenzenesulfonic acid a... 8039477 - Tiagabine. 21763147 - Nsaid-derived γ-secretase modulation requires an acidic moiety on the carbazole scaffold. 2898157 - Effect of 2-pyrrolidone on the concentration of gaba in rat tissues. 16556537 - Design of an i.v. formulation of an unstable prodrug candidate for prostate cancer trea... 15379917 - Efficiency of amino acid utilization in the growing pig at suboptimal levels of intake:... |
Publication Detail:
|
Type: Journal Article Date: 2009-12-07 |
Journal Detail:
|
Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-12-24 Completed Date: 2010-03-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
|
Languages: eng Pagination: 2677-83 Citation Subset: IM |
Affiliation:
|
Technical Research Laboratories, Kyowa Hakko Bio Co., Ltd., Hofu, Yamaguchi, Japan. koichiro.miyake@kyowa-kirin.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biocatalysis* Escherichia coli / cytology, enzymology*, genetics, metabolism Ethylamines / metabolism Glutamate-Cysteine Ligase / biosynthesis, isolation & purification, metabolism* Glutamates / biosynthesis* Hydrogen-Ion Concentration Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Ethylamines; 0/Glutamates; 3081-61-6/theanine; 75-04-7/ethylamine; EC 6.3.2.2/Glutamate-Cysteine Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of Volatile Compounds in Astraeus spp.
Next Document: Immune Deviation and Alleviation of Allergic Reactions in Mice Subjected to Dietary Restriction.