Document Detail


Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection Against Acidity and Protease Activity.
MedLine Citation:
PMID:  22847825     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10(3) active phages after 30 min exposure at pH = 1.6, and protects phage from pepsin activity (4.2 mg mL(-1) ). Non-encapsulated phages are fully inactivated at pH = 1.6 or with pepsin (0.5 mg mL(-1) ) after 10 min.
Authors:
Cecilia Dini; Germán A Islan; Patricio J de Urraza; Guillermo R Castro
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-30
Journal Detail:
Title:  Macromolecular bioscience     Volume:  -     ISSN:  1616-5195     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101135941     Medline TA:  Macromol Biosci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
CIDCA (CONICET-UNLP) CCT La Plata, 1900, La Plata, Argentina; Cátedra de Microbiología, Facultad de Ciencias Exactas, UNLP., 1900, La Plata, Argentina. cdini@biol.unlp.edu.ar.
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